modified_halobacteria_medium_3

Overview

External ontology

mediadive.medium:J1008 (source link unavailable) — MODIFIED HALOBACTERIA MEDIUM-3

Applications

• Microbial cultivation

pH

7.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1008 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented. JCM1008 explicitly states the modified 270 g/L NaCl and 15 g/L magnesium sulfate heptahydrate formulation, corroborated by TOGO M1065's g/L rows. The word supplemented leaves an operational ambiguity: adding magnesium to the unchanged 20 g/L base cannot lower its concentration to 15 g/L. No 35 g/L magnesium or 470 g/L sodium chloride sum is inferred, and this curation does not resolve that preparation ambiguity.

Composition

flowchart LR
    CultureMech_002192["modified_halobacteria_medium_3"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002192 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002192 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002192 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002192 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002192 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_002192 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002192 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002192 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002192 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_distilled_water["distilled water"]
    CultureMech_002192 --> ing9_distilled_water

Ingredients (10)

Preparation

1. Use Medium No. 168 supplemented with 270.0 g/L NaCl (final) and 15.0 g/L MgSO4 x 7H2O. Adjust pH to 7.5.

Target organisms

No target organisms are recorded.

Provenance