Overview
- External ontology
- mediadive.medium:J1089 (source link unavailable) — R AGAR (pH 9.0)
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1089
Composition
flowchart LR
CultureMech_002269["r_agar_ph_9_0"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002269 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002269 --> ing1_Yeast_extract
ing2_Malt_extract["Malt extract"]
CultureMech_002269 --> ing2_Malt_extract
ing3_Casamino_acids["Casamino acids"]
CultureMech_002269 --> ing3_Casamino_acids
ing4_Beef_extract["Beef extract"]
CultureMech_002269 --> ing4_Beef_extract
ing5_Glycerol["Glycerol"]
CultureMech_002269 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Tween_80["Tween 80"]
CultureMech_002269 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002269 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Agar["Agar"]
CultureMech_002269 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
Bacto peptone
|
Copied from referenced JCM Medium 26 |
| 2 | Yeast extract | 5 G_PER_L |
FOODON:03315426
Yeast extract
|
Copied from referenced JCM Medium 26 |
| 3 | Malt extract | 5 G_PER_L |
FOODON:03301056
Malt extract
|
Copied from referenced JCM Medium 26 |
| 4 | Casamino acids | 5 G_PER_L |
FOODON:03315719
Casamino acids
|
Copied from referenced JCM Medium 26 |
| 5 | Beef extract | 2 G_PER_L |
FOODON:03302088
Beef extract
|
Copied from referenced JCM Medium 26 |
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
Copied from referenced JCM Medium 26 |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.05 G_PER_L |
CHEBI:53426
polysorbate 80
|
Copied from referenced JCM Medium 26 |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Copied from referenced JCM Medium 26 |
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 26 |
Preparation
- Prepare R agar (see Medium No. 26). After autoclaving, adjust pH to 9.0 with sterilized 10% Na2CO3 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.590784Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1089 |
| 2026-02-03T11:26:52.897405 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:11.811584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002269 |
| 2026-03-17T19:45:24.682494+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R AGAR (pH 9.0)" to "r_agar_ph_9_0" for consistent matching |
| 2026-04-04T21:34:20.692015 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from r_agar (CultureMech:002627) |
| 2026-04-04T21:40:32.859003 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from r_agar (CultureMech:002627) |
| 2026-04-04T21:48:41.096300 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from r_agar (CultureMech:002627) |
| 2026-05-16T08:58:13.883508+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:43.751104+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.076500+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch6.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 | Applied local mappings for Bacto peptone, Beef extract, Casamino acids, Malt extract, Yeast extract. |