Overview
- External ontology
- mediadive.medium:J409 (source link unavailable) — HORIKOSHI-I MEDIUM WITH 2% NaCl
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 409 defines Horikoshi-I Medium with 2% NaCl as 1 L of JCM Medium 181 Horikoshi-I medium plus 20 g/L NaCl.
Composition
flowchart LR
CultureMech_002765["horikoshi_i_medium_with_2_nacl"]
ing0_NaCl["NaCl"]
CultureMech_002765 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Adds 20 g/L NaCl to JCM Medium 181 Horikoshi-I medium. |
Preparation
- Adds 20 g/L NaCl to JCM Medium 181 Horikoshi-I medium.
- Autoclave at 121 C for 15 min.
Solutions
Horikoshi-I medium
Dose: 1000 ML_PER_L
Adds 20 g/L NaCl to JCM Medium 181 Horikoshi-I medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.534738Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J409 |
| 2026-03-13T06:19:15.569952Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002765 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.984890+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HORIKOSHI-I MEDIUM WITH 2% NaCl" to "horikoshi_i_medium_with_2_nacl" for consistent matching |
| 2026-06-05T07:41:08.705051+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-08T00:00:00-07:00 | repair_jcm_salinity_score20.py | RESOLVED_JCM_SALINITY_SCORE20 | Adds 20 g/L NaCl to JCM Medium 181 Horikoshi-I medium. |