acidic_glycerol_asparagine_agar_ph_5_0

CultureMech:002775 bacterial COMPLEX LIQUID
Original name: ACIDIC GLYCEROL-ASPARAGINE AGAR (pH 5.0)

Overview

External ontology
mediadive.medium:J422 (source link unavailable) — ACIDIC GLYCEROL-ASPARAGINE AGAR (pH 5.0)
Applications
  • Microbial cultivation
pH
5.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=422

Composition

flowchart LR
    CultureMech_002775["acidic_glycerol_asparagine_agar_ph_5_0"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_002775 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Glycerol["Glycerol"]
    CultureMech_002775 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002775 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar["Agar"]
    CultureMech_002775 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 L-Asparagine
C4H8N2O3 132.119 g/mol
0.999001 G_PER_L CHEBI:17196
L-asparagine
 Copied from referenced JCM Medium 60
2 Glycerol
C3H8O3 92.094 g/mol
9.99001 G_PER_L CHEBI:17754
glycerol
 Copied from referenced JCM Medium 60
3 K2HPO4
HO4P.2K 174.174 g/mol
0.999001 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Copied from referenced JCM Medium 60
4 Agar 14.985 G_PER_L CHEBI:2509
agar
 Copied from referenced JCM Medium 60

Preparation

  1. Prepare Glycerol-asparagine agar (ISP-5) (see Medium No. 60) and adjust pH to 5.0.

Solutions

Trace salt solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L Copied from referenced JCM Medium 60
MnCl2 x 4 H2O 1 G_PER_L Copied from referenced JCM Medium 60
ZnSO4 x 7 H2O 1 G_PER_L Copied from referenced JCM Medium 60

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.575976Z mediadive-import Imported from MediaDive Source: JCM, ID: J422
2026-02-03T11:26:54.882653 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:15.650338Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002775
2026-03-17T19:45:29.075265+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIC GLYCEROL-ASPARAGINE AGAR (pH 5.0)" to "acidic_glycerol_asparagine_agar_ph_5_0" for consistent matching
2026-04-04T21:48:41.211245 resolve_option_c_references RESOLVED_REFERENCE Resolved reference by copying from glycerol_asparagine_agar_isp_5 (CultureMech:002957)
2026-04-04T21:54:30.554720 resolve_option_c_references RESOLVED_REFERENCE Resolved reference by copying from glycerol_asparagine_agar_isp_5 (CultureMech:002957)
2026-05-16T08:57:22.138919+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:40:47.126748+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.531224+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T07:02:12.429696+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).