modified_halobacteria_medium (CultureMech:002920)

Overview

External ontology

mediadive.medium:J572 (source link unavailable) — MODIFIED HALOBACTERIA MEDIUM

Applications

Microbial cultivation

pH

7.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=572 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented. The inherited base pH range does not replace the source-explicit post-autoclave pH 7.5 target.

Composition

flowchart LR
    CultureMech_002920["modified_halobacteria_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002920 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002920 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002920 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002920 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002920 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_002920 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002920 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002920 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002920 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_distilled_water["distilled water"]
    CultureMech_002920 --> ing9_distilled_water

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Casamino acids	5 G_PER_L		Copied from referenced JCM Medium 168 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
2	Yeast extract	5 G_PER_L		Copied from referenced JCM Medium 168 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
3	Na glutamate C5H8NO4.Na 169.112 g/mol	1 G_PER_L	CHEBI:64220 monosodium glutamate	Copied from referenced JCM Medium 168 1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
4	Trisodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate	Copied from referenced JCM Medium 168 3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Copied from referenced JCM Medium 168 Use the recipe-specific final formulation, not the copied base amount. The source explicitly gives this final g/L target.
6	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride	Copied from referenced JCM Medium 168 2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
7	NaCl Cl.Na 58.443 g/mol	200 G_PER_L	CHEBI:26710 sodium chloride	Copied from referenced JCM Medium 168 200.0 g in the source preparation brought to 1.0 L gives 200 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
8	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	36 MG_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Copied from referenced JCM Medium 168 36.0 mg in the source preparation brought to 1.0 L gives 36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
9	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 MG_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Copied from referenced JCM Medium 168 0.36 mg in the source preparation brought to 1.0 L gives 0.36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
10	distilled water			Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported.

Preparation

Use Medium No. 168 with 2.0 g/L MgSO4 x 7H2O (final). After autoclaving, adjust pH to 7.5 with sterile Na2CO3 solution.

Solutions

Na2CO3 solution

Sterile sodium carbonate solution adjusts the medium to pH 7.5 after autoclaving. Dose and stock strength are unspecified; retain any source-explicit variable dose without inventing a numeric concentration.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.969698Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J572
2026-02-03T11:26:55.360246	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:16.570744Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002920
2026-03-17T19:45:30.139029+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MODIFIED HALOBACTERIA MEDIUM" to "modified_halobacteria_medium" for consistent matching
2026-04-04T18:49:19.149921	copy_referenced_compositions	RESOLVED_REFERENCE	Resolved reference by copying from halobacteria_medium (CultureMech:002527)
2026-05-16T08:58:09.554397+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:36.668076+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.792603+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-09T14:23:51.976655+00:00	codex	CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES	Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added.