Overview
- External ontology
- mediadive.medium:J617 (source link unavailable) — MODIFIED PY4S AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=617
Composition
flowchart LR
CultureMech_002964["modified_py4s_agar"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002964 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002964 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_002964 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_002964 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_002964 --> ing4_Maltose
CHEBI_17306["CHEBI:17306"]
ing4_Maltose -.-> CHEBI_17306
ing5_Starch["Starch"]
CultureMech_002964 --> ing5_Starch
CHEBI_28017["CHEBI:28017"]
ing5_Starch -.-> CHEBI_28017
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_002964 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_Na2CO3["Na2CO3"]
CultureMech_002964 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002964 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Agar["Agar"]
CultureMech_002964 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 9.96016 G_PER_L | Copied from referenced JCM Medium 363 | |
| 2 | Yeast extract | 4.98008 G_PER_L | Copied from referenced JCM Medium 363 | |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.249004 G_PER_L |
CHEBI:17234
glucose
|
Copied from referenced JCM Medium 363 |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.249004 G_PER_L |
CHEBI:17057
cellobiose
|
Copied from referenced JCM Medium 363 |
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
0.249004 G_PER_L |
CHEBI:17306
maltose
|
Copied from referenced JCM Medium 363 |
| 6 | Starch | 0.249004 G_PER_L |
CHEBI:28017
starch
|
Copied from referenced JCM Medium 363 |
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 363 |
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
Copied from referenced JCM Medium 363 |
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.298805 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Copied from referenced JCM Medium 363 |
| 10 | Agar | 14.9402 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 363 |
Preparation
- Use Medium No. 363 supplemented with 5 mg/L hemin and 10 �g/L vitamin B12. Adjust pH to 7.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.100357Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J617 |
| 2026-02-03T11:26:55.504674 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:16.853188Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002964 |
| 2026-03-17T19:45:30.465296+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED PY4S AGAR" to "modified_py4s_agar" for consistent matching |
| 2026-04-04T18:49:19.523325 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from py4s_agar (CultureMech:002722) |
| 2026-05-16T08:58:10.166858+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:37.641010+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.915744+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |