modified_thermus_medium_with_3_nacl (CultureMech:002970)

Overview

External ontology

mediadive.medium:J624 (source link unavailable) — MODIFIED THERMUS MEDIUM WITH 3% NaCl

Applications

Microbial cultivation

pH

7.8

Notes

JCM Medium 624 Modified Thermus Medium with 3% NaCl lists 3.0 g Peptone, 1.0 g Yeast extract, 1.0 g Sodium glutamate-H2O, 30.0 g NaCl, 10.0 ml Castenholz basal salt solution from JCM Medium 273, and 1.0 L Distilled water, then adjusts pH to 7.8.

Composition

flowchart LR
    CultureMech_002970["modified_thermus_medium_with_3_nacl"]
    ing0_Peptone["Peptone"]
    CultureMech_002970 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002970 --> ing1_Yeast_extract
    ing2_Sodium_glutamate_x_H2O["Sodium glutamate x H2O"]
    CultureMech_002970 --> ing2_Sodium_glutamate_x_H2O
    CHEBI_232425["CHEBI:232425"]
    ing2_Sodium_glutamate_x_H2O -.-> CHEBI_232425
    ing3_NaCl["NaCl"]
    CultureMech_002970 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Distilled_water["Distilled water"]
    CultureMech_002970 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	3.0 G_PER_L	MICRO:0000178 (source link unavailable) peptone	MediaDive J624 / JCM Medium 624 lists 3.0 g/L Peptone.
2	Yeast extract	1.0 G_PER_L	FOODON:03315426 yeast extract	MediaDive J624 / JCM Medium 624 lists 1.0 g/L Yeast extract.
3	Sodium glutamate x H2O C5H8NO4.H2O.Na 187.127 g/mol	1.0 G_PER_L	CHEBI:232425 monosodium L-glutamate hydrate	MediaDive J624 / JCM Medium 624 lists 1.0 g/L Sodium glutamate x H2O.
4	NaCl Cl.Na 58.443 g/mol	30.0 G_PER_L	CHEBI:26710 sodium chloride	MediaDive J624 / JCM Medium 624 lists 30.0 g/L NaCl.
5	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	MediaDive J624 / JCM Medium 624 lists 1.0 L Distilled water.

Preparation

Add the base components and 10.0 ml Castenholz basal salt solution to 1.0 L distilled water.
Adjust pH to 7.8.

Solutions

Castenholz basal salt solution (see Medium No. 273)

Dose: 10.0 ML_PER_L

CultureMech:013022

Stock composition
Ingredient	Concentration	Notes
Nitrilotriacetic acid	1.0 G_PER_L	JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid.
CaSO4 x 2 H2O	0.6 G_PER_L	JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O.
MgSO4 x 7 H2O	1.0 G_PER_L	JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O.
NaCl	0.08 G_PER_L	JCM Medium 273 lists 0.08 g/L NaCl.
KNO3	1.03 G_PER_L	JCM Medium 273 lists 1.03 g/L KNO3.
NaNO3	6.89 G_PER_L	JCM Medium 273 lists 6.89 g/L NaNO3.
Na2HPO4	1.11 G_PER_L	JCM Medium 273 lists 1.11 g/L Na2HPO4.
FeCl3 x 6 H2O solution (0.03%)	10.0 ML_PER_L	JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution (0.03%).
Nitsch's trace elements	10.0 ML_PER_L	JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.
Distilled water	1.0 L	JCM Medium 273 lists 1.0 L Distilled water in the stock solution.

JCM Medium 624 adds 10.0 ml/L Castenholz basal salt solution from JCM Medium 273.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.121147Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J624
2026-03-13T03:08:47.714848Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:16.892195Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002970
2026-03-17T06:29:50.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.508360+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MODIFIED THERMUS MEDIUM WITH 3% NaCl" to "modified_thermus_medium_with_3_nacl" for consistent matching
2026-06-05T07:41:37.925801+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.665243+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:09.007175+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m634_m635_score15.py	RESOLVED_JCM_624_THERMUS_3_NACL	Restored the printed JCM Medium 624 base recipe, represented Castenholz basal salt solution as a 10.0 ml/L stock from JCM Medium 273 instead of flattening stock concentrations into the final medium, grounded the disclosed solutes, and linked TOGO M634 and M635.