Overview
- External ontology
- mediadive.medium:J777 (source link unavailable) — MH1 MEDIUM FOR SALIARCHAEUM ACIDOPHILUM
- Applications
-
- Microbial cultivation
- pH
- 4.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=777
Composition
flowchart LR
CultureMech_003119["mh1_medium_for_saliarchaeum_acidophilum"]
ing0_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_003119 --> ing0_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamic_acid -.-> CHEBI_16015
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003119 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_KH2PO4["KH2PO4"]
CultureMech_003119 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_003119 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2SO4["K2SO4"]
CultureMech_003119 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003119 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_NaCl["NaCl"]
CultureMech_003119 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Casamino_acids["Casamino acids"]
CultureMech_003119 --> ing7_Casamino_acids
ing8_Yeast_extract["Yeast extract"]
CultureMech_003119 --> ing8_Yeast_extract
ing9_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
CultureMech_003119 --> ing9_FeSO4_x_6_H2O
CHEBI_75832["CHEBI:75832"]
ing9_FeSO4_x_6_H2O -.-> CHEBI_75832
ing10_Agar["Agar"]
CultureMech_003119 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003119 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003119 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnCl2["ZnCl2"]
CultureMech_003119 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_H3BO4["H3BO4"]
CultureMech_003119 --> ing14_H3BO4
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003119 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003119 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003119 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_AlCl3["AlCl3"]
CultureMech_003119 --> ing18_AlCl3
CHEBI_30114["CHEBI:30114"]
ing18_AlCl3 -.-> CHEBI_30114
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003119 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003119 --> ing20_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
ing21_BaCl2_x_2_H2O["BaCl2 x 2 H2O"]
CultureMech_003119 --> ing21_BaCl2_x_2_H2O
CHEBI_86153["CHEBI:86153"]
ing21_BaCl2_x_2_H2O -.-> CHEBI_86153
ing22_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_003119 --> ing22_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing22_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
2 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Copied from referenced JCM Medium 776 |
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2 G_PER_L |
CHEBI:53258
sodium citrate
|
Copied from referenced JCM Medium 776 |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 776 |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 776 |
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
Copied from referenced JCM Medium 776 |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 776 |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
250.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 200.0, 50.0] | Copied from JCM Medium 776 |
| 8 | Casamino acids | 4 G_PER_L | Copied from referenced JCM Medium 776 | |
| 9 | Yeast extract | 2 G_PER_L | Copied from referenced JCM Medium 776 | |
| 10 |
FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
|
0.004 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Copied from referenced JCM Medium 776 |
| 11 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 776 |
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.3 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Copied from referenced JCM Medium 776 |
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 776 |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Copied from referenced JCM Medium 776 |
| 15 | H3BO4 | 0.02 G_PER_L | Copied from referenced JCM Medium 776 | |
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 776 |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
Copied from referenced JCM Medium 776 |
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Copied from referenced JCM Medium 776 |
| 19 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Copied from referenced JCM Medium 776 |
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Copied from referenced JCM Medium 776 |
| 21 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Copied from referenced JCM Medium 776 |
| 22 |
BaCl2 x 2 H2O
Ba.2Cl.2H2O 244.264 g/mol
|
0.1 G_PER_L |
CHEBI:86153
barium chloride dihydrate
|
Copied from referenced JCM Medium 776 |
| 23 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Copied from referenced JCM Medium 776 |
Preparation
- Use Medium No. 776 with 2.0 g/L MgCl2 x 6H2O (final) and adjust pH to 4.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.568212Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J777 |
| 2026-02-03T11:26:56.063951 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:17.949824Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003119 |
| 2026-03-17T19:45:31.741049+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MH1 MEDIUM FOR SALIARCHAEUM ACIDOPHILUM" to "mh1_medium_for_saliarchaeum_acidophilum" for consistent matching |
| 2026-04-04T18:49:20.037954 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from mh1_medium_for_saliarchaeum_rubrodurum (CultureMech:003118) |
| 2026-05-16T08:58:07.734629+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:33.651361+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.122529+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:07.757147+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |