Overview
- External ontology
- mediadive.medium:J910 (source link unavailable) — MODIFIED SAP2 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=910
Composition
flowchart LR
CultureMech_003259["modified_sap2_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_003259 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003259 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_003259 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_NaCl["NaCl"]
CultureMech_003259 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_003259 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003259 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003259 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003259 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 1 G_PER_L | Copied from referenced JCM Medium 651 | |
| 2 | Yeast extract | 1 G_PER_L | Copied from referenced JCM Medium 651 | |
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 651 |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
Copied from referenced JCM Medium 651 |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.35 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 651 |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 651 |
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Copied from referenced JCM Medium 651 |
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Copied from referenced JCM Medium 651 |
Preparation
- Use Medium No. 651 with 1.0 x artificial seawater (instead of the 0.5 x artificial seawater). For preparation of liquid medium, omit agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.981058Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J910 |
| 2026-02-03T11:26:56.585802 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:18.999430Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003259 |
| 2026-03-17T19:45:32.900882+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED SAP2 MEDIUM" to "modified_sap2_medium" for consistent matching |
| 2026-04-04T18:49:19.921272 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from sap2_medium (CultureMech:002997) |
| 2026-05-16T08:58:10.216648+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:37.708978+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |