halobacteria_medium_with_15_nacl

Overview

External ontology

mediadive.medium:J914 (source link unavailable) — HALOBACTERIA MEDIUM WITH 15% NaCl

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=914 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented.

Composition

flowchart LR
    CultureMech_003262["halobacteria_medium_with_15_nacl"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_003262 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003262 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_003262 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_003262 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003262 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_003262 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_003262 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003262 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003262 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_distilled_water["distilled water"]
    CultureMech_003262 --> ing9_distilled_water

Ingredients (10)

Preparation

1. Use Medium No. 168 with 150.0 g/L (final) NaCl. Omit agar for preparation of liquid medium.

Target organisms

No target organisms are recorded.

Provenance