methanococcoides_medium

CultureMech:007554 archaea COMPLEX LIQUID
Original name: Methanococcoides Medium

Overview

External ontology
TOGO:M1039 — Methanococcoides Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1039 Original source: JCM - JCM_M986 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=986

Composition

flowchart LR
    CultureMech_007554["methanococcoides_medium"]
    ing0_trimethylamine_HCl["trimethylamine・HCl"]
    CultureMech_007554 --> ing0_trimethylamine_HCl
    ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007554 --> ing1_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing1_Carbon_dioxide_gas -.-> CHEBI_16526
    ing2_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007554 --> ing2_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing2_Nitrogen_gas -.-> CHEBI_17997

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 trimethylamine・HCl 3 G_PER_L Role: Nutrient source; Properties: Defined component, Organic compound, Simple component
2 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
3 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Preparation

  1. Use Medium No. [265] supplemented with 3.0 g/L trimethylamine・HCl and adjust pH to 7.0 -- 7.2. Prepare the medium and cultivate under a N2--CO2(80:20, v/v) gas mixture.

Solutions

METHANOGENIUM MEDIUM (see Medium [M257])

Dose: 1 L

CultureMech:009148

Cross-reference to Medium M257 TOGO specifies 1 L of referenced M257 base medium as a recipe input, not 1 g/L or a measured final-medium volume. This dose is dataset-specific; inherited base concentrations remain unverified.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.806337Z togo-import Imported from TOGO Medium Source: JCM, ID: M1039
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:38.453724+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:49.624888Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007554
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T19:46:07.972630+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanococcoides Medium" to "methanococcoides_medium" for consistent matching
2026-05-16T09:03:07.094540+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-10-08T10:47:19.502771+00:00 codex REPAIRED_METHANOGEN_STOCK_DOSES_AND_BASE_REFERENCE_WITH_EVIDENCE Corrected recipe-specific quantities and provenance; preserved stock-strength conflicts and unresolved final-volume/base-composition claims. Existing history, conditions and organism claims unchanged.