Overview
- External ontology
- TOGO:M1059 — Acidic HB-1 Medium
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- JCM Medium 1003 acidifies JCM Medium 744 to pH 5.5 before autoclaving and then adds 10% KNO3, 20% MES at pH 5.5, and neutralized 3% Na2S x 9H2O aseptically and anaerobically.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare JCM Medium 744 and adjust pH to 5.5 before autoclaving.
- Autoclave at 121 C for 15 min.
- Aseptically and anaerobically add 10% KNO3, 20% MES, and neutralized 3% Na2S x 9H2O from anaerobic stocks.
Solutions
Modified HB-1 Medium
Dose: 1000 ML_PER_L
JCM Medium 1003 uses JCM Medium 744 as the base medium and adjusts it to pH 5.5 before autoclaving.
10% KNO3 solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KNO3 | 10 PERCENT_W_V | JCM Medium 1003 identifies this as a 10% stock solution. |
JCM Medium 1003 lists 20.0 ml/L 10% KNO3 solution.
20% MES (pH 5.5) solution
Dose: 25.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MES | 20 PERCENT_W_V | JCM Medium 1003 identifies this as a 20% stock solution. |
JCM Medium 1003 lists 25.0 ml/L 20% MES (pH 5.5) solution.
3% Na2S x 9H2O solution (neutralized)
Dose: 4.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 3 PERCENT_W_V | JCM Medium 1003 identifies this as a 3% stock solution. |
JCM Medium 1003 lists 4.0 ml/L 3% Na2S x 9H2O solution (neutralized).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.843035Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1059 |
| 2026-03-09T08:04:41.821203+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:49.786134Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007575 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:08.137490+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidic HB-1 Medium" to "acidic_hb_1_medium" for consistent matching |
| 2026-05-16T09:03:07.257876+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-07T00:00:00-07:00 | repair_cross_reference_score25.py | RESOLVED_SCORE25_CROSS_REFERENCE | JCM Medium 1003 acidifies JCM Medium 744 to pH 5.5 before autoclaving and then adds 10% KNO3, 20% MES at pH 5.5, and neutralized 3% Na2S x 9H2O aseptically and anaerobically. |