r_agar_with_0_5_tween_80

CultureMech:009502 bacterial COMPLEX SOLID_AGAR
Original name: R Agar With 0.5% Tween 80

Overview

External ontology
TOGO:M297 — R Agar With 0.5% Tween 80
Applications
  • Microbial cultivation
Notes
TOGO Medium M297 mirrors JCM Medium 302: 1 L of TOGO M19/JCM Medium 26 R Agar plus 5 g/L Tween 80.

Composition

flowchart LR
    CultureMech_009502["r_agar_with_0_5_tween_80"]
    ing0_Tween_80["Tween 80"]
    CultureMech_009502 --> ing0_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing0_Tween_80 -.-> CHEBI_53426

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
5 G_PER_L CHEBI:53426
polysorbate 80
 Adds 5 g/L Tween 80 to TOGO M19 R Agar.

Preparation

  1. Adds 5 g/L Tween 80 to TOGO M19 R Agar.
  2. Autoclave at 121 C for 15 min.

Solutions

R agar

Dose: 1000 ML_PER_L

CultureMech:008585

Adds 5 g/L Tween 80 to TOGO M19 R Agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.790950Z togo-import Imported from TOGO Medium Source: JCM, ID: M297
2026-01-28T19:48:38.426320Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:04.288334+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:06.434286Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009502
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.435272+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R Agar With 0.5% Tween 80" to "r_agar_with_0_5_tween_80" for consistent matching
2026-05-16T09:03:16.313908+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:39.577902+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py RESOLVED_R_AGAR_SALINITY_SCORE20 Adds 5 g/L Tween 80 to TOGO M19 R Agar.