thermococcus_medium_ph_6_0

CultureMech:009724 archaea COMPLEX LIQUID
Original name: Thermococcus Medium (pH 6.0)

Overview

External ontology
TOGO:M345 — Thermococcus Medium (pH 6.0)
Applications
  • Microbial cultivation
pH
6.0
Notes
TOGO M345 uses one liter of TOGO M273 Thermococcus Medium and readjusts the reduced medium to pH 6.0 with 1.0 N H2SO4.

Composition

flowchart LR
    CultureMech_009724["thermococcus_medium_ph_6_0"]
    ing0_H2SO4["H2SO4"]
    CultureMech_009724 --> ing0_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing0_H2SO4 -.-> CHEBI_26836

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 H2SO4
H2O4S 98.079 g/mol
1 N VARIABLE CHEBI:26836
sulfuric acid
 TOGO lists 1 N H2SO4 for final pH adjustment. Normality is retained verbatim because the schema has no normality unit.

Preparation

  1. Use one liter of Thermococcus Medium from TOGO M273 and, after reduction, readjust to pH 6.0 with 1.0 N H2SO4.

Solutions

Thermococcus medium (see Medium [M273])

Dose: 1 L

CultureMech:009290

TOGO M345 uses one liter of TOGO M273 Thermococcus Medium as the base medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.882013Z togo-import Imported from TOGO Medium Source: JCM, ID: M345
2026-01-28T19:48:17.781334Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-09T08:04:29.454449+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.373875Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009724
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.363114+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Thermococcus Medium (pH 6.0)" to "thermococcus_medium_ph_6_0" for consistent matching
2026-05-16T09:03:17.268558+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.575108+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_archaea_ph_wrappers_score20.py RESOLVED_ARCHAEA_PH_WRAPPER_SCORE20 Readjusts TOGO M273 Thermococcus Medium to pH 6.0 with 1.0 N H2SO4 after reduction.