horikoshi_i_medium_with_2_nacl

CultureMech:009791 bacterial COMPLEX SOLID_AGAR
Original name: Horikoshi-I Medium With 2% NaCl

Overview

External ontology
TOGO:M407 — Horikoshi-I Medium With 2% NaCl
Applications
  • Microbial cultivation
Notes
TOGO Medium M407 records Horikoshi-I Medium with 2% NaCl as 1 L of TOGO M174/JCM Medium 181 Horikoshi-I medium plus 20 g/L NaCl.

Composition

flowchart LR
    CultureMech_009791["horikoshi_i_medium_with_2_nacl"]
    ing0_NaCl["NaCl"]
    CultureMech_009791 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Adds 20 g/L NaCl to TOGO M174/JCM Medium 181 Horikoshi-I medium.

Preparation

  1. Adds 20 g/L NaCl to TOGO M174/JCM Medium 181 Horikoshi-I medium.
  2. Autoclave at 121 C for 15 min.

Solutions

Horikoshi-I medium

Dose: 1000 ML_PER_L

CultureMech:008313

Adds 20 g/L NaCl to TOGO M174/JCM Medium 181 Horikoshi-I medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.999625Z togo-import Imported from TOGO Medium Source: JCM, ID: M407
2026-01-28T19:48:22.450115Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:37.474655+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.905685Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009791
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.897638+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Horikoshi-I Medium With 2% NaCl" to "horikoshi_i_medium_with_2_nacl" for consistent matching
2026-05-16T09:03:17.782240+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:41.132672+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py RESOLVED_JCM_SALINITY_SCORE20 Adds 20 g/L NaCl to TOGO M174/JCM Medium 181 Horikoshi-I medium.