glycerol_asparagine_agar_isp_5_with_10_nacl

CultureMech:009792 bacterial COMPLEX SOLID_AGAR
Original name: Glycerol-Asparagine Agar (ISP-5) With 10% NaCl

Overview

External ontology
TOGO:M408 — Glycerol-Asparagine Agar (ISP-5) With 10% NaCl
Applications
  • Microbial cultivation
Notes
TOGO Medium M408 records JCM Medium 410: 1 L of TOGO M52/JCM Medium 60 Glycerol-Asparagine Agar (ISP-5) plus 100 g/L NaCl.

Composition

flowchart LR
    CultureMech_009792["glycerol_asparagine_agar_isp_5_with_10_nacl"]
    ing0_NaCl["NaCl"]
    CultureMech_009792 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
 Adds 100 g/L NaCl to TOGO M52/JCM Medium 60 Glycerol-Asparagine Agar (ISP-5).

Preparation

  1. Adds 100 g/L NaCl to TOGO M52/JCM Medium 60 Glycerol-Asparagine Agar (ISP-5).
  2. Autoclave at 121 C for 15 min.

Solutions

Glycerol-Asparagine Agar (ISP-5)

Dose: 1000 ML_PER_L

CultureMech:009922

Adds 100 g/L NaCl to TOGO M52/JCM Medium 60 Glycerol-Asparagine Agar (ISP-5).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.000329Z togo-import Imported from TOGO Medium Source: JCM, ID: M408
2026-01-28T19:48:23.516269Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:39.096561+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.908944Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009792
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.901021+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Glycerol-Asparagine Agar (ISP-5) With 10% NaCl" to "glycerol_asparagine_agar_isp_5_with_10_nacl" for consistent matching
2026-05-16T09:03:17.786334+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:41.136731+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-08T00:00:00-07:00 repair_jcm_isp5_salinity_score20.py RESOLVED_JCM_ISP5_SALINITY_SCORE20 Adds 100 g/L NaCl to TOGO M52/JCM Medium 60 Glycerol-Asparagine Agar (ISP-5).