Overview
- External ontology
- TOGO:M634 — Modified Thermus Medium With 3% NaCl
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- JCM Medium 624 Modified Thermus Medium with 3% NaCl lists 3.0 g Peptone, 1.0 g Yeast extract, 1.0 g Sodium glutamate-H2O, 30.0 g NaCl, 10.0 ml Castenholz basal salt solution from JCM Medium 273, and 1.0 L Distilled water, then adjusts pH to 7.8.
Composition
flowchart LR
CultureMech_010034["modified_thermus_medium_with_3_nacl"]
ing0_Peptone["Peptone"]
CultureMech_010034 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_010034 --> ing1_Yeast_extract
ing2_Sodium_glutamate_x_H2O["Sodium glutamate x H2O"]
CultureMech_010034 --> ing2_Sodium_glutamate_x_H2O
CHEBI_232425["CHEBI:232425"]
ing2_Sodium_glutamate_x_H2O -.-> CHEBI_232425
ing3_NaCl["NaCl"]
CultureMech_010034 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Distilled_water["Distilled water"]
CultureMech_010034 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 3.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
TOGO M634 / JCM Medium 624 lists 3.0 g/L Peptone. |
| 2 | Yeast extract | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M634 / JCM Medium 624 lists 1.0 g/L Yeast extract. |
| 3 |
Sodium glutamate x H2O
C5H8NO4.H2O.Na 187.127 g/mol
|
1.0 G_PER_L |
CHEBI:232425
monosodium L-glutamate hydrate
|
TOGO M634 / JCM Medium 624 lists 1.0 g/L Sodium glutamate x H2O. |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
30.0 G_PER_L |
CHEBI:26710
sodium chloride
|
TOGO M634 / JCM Medium 624 lists 30.0 g/L NaCl. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M634 / JCM Medium 624 lists 1.0 L Distilled water. |
Preparation
- Add the base components and 10.0 ml Castenholz basal salt solution to 1.0 L distilled water.
- Adjust pH to 7.8.
Solutions
Castenholz basal salt solution (see Medium No. 273)
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.0 G_PER_L | JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid. |
| CaSO4 x 2 H2O | 0.6 G_PER_L | JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O. |
| MgSO4 x 7 H2O | 1.0 G_PER_L | JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O. |
| NaCl | 0.08 G_PER_L | JCM Medium 273 lists 0.08 g/L NaCl. |
| KNO3 | 1.03 G_PER_L | JCM Medium 273 lists 1.03 g/L KNO3. |
| NaNO3 | 6.89 G_PER_L | JCM Medium 273 lists 6.89 g/L NaNO3. |
| Na2HPO4 | 1.11 G_PER_L | JCM Medium 273 lists 1.11 g/L Na2HPO4. |
| FeCl3 x 6 H2O solution (0.03%) | 10.0 ML_PER_L | JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution (0.03%). |
| Nitsch's trace elements | 10.0 ML_PER_L | JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements. |
| Distilled water | 1.0 L | JCM Medium 273 lists 1.0 L Distilled water in the stock solution. |
JCM Medium 624 adds 10.0 ml/L Castenholz basal salt solution from JCM Medium 273.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.439267Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M634 |
| 2026-01-28T19:48:25.952416Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:43.044442+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:10.852121Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010034 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.779375+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Thermus Medium With 3% NaCl" to "modified_thermus_medium_with_3_nacl" for consistent matching |
| 2026-05-16T09:03:19.643044+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.135766+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m634_m635_score15.py | RESOLVED_TOGO_M634_SCORE15 | Corrected the imported distilled-water and Castenholz-stock units, added pH 7.8, grounded Peptone, Yeast extract, and Sodium glutamate-H2O, linked the JCM 624 source duplicate, and added the existing TOGO M636 supplemented child backlink. |