modified_thermus_medium_with_3_nacl

CultureMech:010035 bacterial COMPLEX SOLID_AGAR
Original name: Modified Thermus Medium With 3% NaCl

Overview

External ontology
TOGO:M635 — Modified Thermus Medium With 3% NaCl
Applications
  • Microbial cultivation
pH
7.8
Notes
JCM Medium 624 Modified Thermus Medium with 3% NaCl lists the same liquid base and directs agar plates to be prepared with 20.0 g/L agar.

Composition

flowchart LR
    CultureMech_010035["modified_thermus_medium_with_3_nacl"]
    ing0_Peptone["Peptone"]
    CultureMech_010035 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010035 --> ing1_Yeast_extract
    ing2_Sodium_glutamate_x_H2O["Sodium glutamate x H2O"]
    CultureMech_010035 --> ing2_Sodium_glutamate_x_H2O
    CHEBI_232425["CHEBI:232425"]
    ing2_Sodium_glutamate_x_H2O -.-> CHEBI_232425
    ing3_NaCl["NaCl"]
    CultureMech_010035 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Distilled_water["Distilled water"]
    CultureMech_010035 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar["Agar"]
    CultureMech_010035 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Peptone 3.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 TOGO M635 / JCM Medium 624 agar plate lists 3.0 g/L Peptone.
2 Yeast extract 1.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M635 / JCM Medium 624 agar plate lists 1.0 g/L Yeast extract.
3 Sodium glutamate x H2O
C5H8NO4.H2O.Na 187.127 g/mol
1.0 G_PER_L CHEBI:232425
monosodium L-glutamate hydrate
 TOGO M635 / JCM Medium 624 agar plate lists 1.0 g/L Sodium glutamate x H2O.
4 NaCl
Cl.Na 58.443 g/mol
30.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M635 / JCM Medium 624 agar plate lists 30.0 g/L NaCl.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M635 / JCM Medium 624 agar plate lists 1.0 L Distilled water.
6 Agar 20.0 G_PER_L CHEBI:2509
agar
 JCM Medium 624 instructs agar plates to add 20.0 g/L agar.

Preparation

  1. Add the base components and 10.0 ml Castenholz basal salt solution to 1.0 L distilled water.
  2. Adjust pH to 7.8.
  3. For agar plates, add 20.0 g/L agar.

Solutions

Castenholz basal salt solution (see Medium No. 273)

Dose: 10.0 ML_PER_L

CultureMech:013022

Stock composition
IngredientConcentrationNotes
Nitrilotriacetic acid 1.0 G_PER_L JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid.
CaSO4 x 2 H2O 0.6 G_PER_L JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O.
MgSO4 x 7 H2O 1.0 G_PER_L JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O.
NaCl 0.08 G_PER_L JCM Medium 273 lists 0.08 g/L NaCl.
KNO3 1.03 G_PER_L JCM Medium 273 lists 1.03 g/L KNO3.
NaNO3 6.89 G_PER_L JCM Medium 273 lists 6.89 g/L NaNO3.
Na2HPO4 1.11 G_PER_L JCM Medium 273 lists 1.11 g/L Na2HPO4.
FeCl3 x 6 H2O solution (0.03%) 10.0 ML_PER_L JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution (0.03%).
Nitsch's trace elements 10.0 ML_PER_L JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.
Distilled water 1.0 L JCM Medium 273 lists 1.0 L Distilled water in the stock solution.
JCM Medium 624 adds 10.0 ml/L Castenholz basal salt solution from JCM Medium 273.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.440309Z togo-import Imported from TOGO Medium Source: JCM, ID: M635
2026-01-28T19:48:13.938774Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:23.118965+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:10.856909Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010035
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.784404+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Thermus Medium With 3% NaCl" to "modified_thermus_medium_with_3_nacl" for consistent matching
2026-05-16T09:03:19.648489+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:43.141631+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m634_m635_score15.py RESOLVED_TOGO_M635_SCORE15 Corrected the imported distilled-water and Castenholz-stock units, added pH 7.8, grounded Peptone, Yeast extract, Sodium glutamate-H2O, and Agar, represented M635 as the JCM Medium 624 agar-plate variant, and linked the JCM 624 parent.