All families / PF00563
PF00563: EAL
EAL domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF00563: EAL domain
IPR001633: integrated entry for EAL domain
Source description
This domain is found in diverse bacterial signaling proteins. It is called EAL after its conserved residues. The EAL domain is a good candidate for a diguanylate phosphodiesterase function [InterPro reference PUB00007150]. The domain contains many conserved acidic residues that could participate in metal binding and might form the phosphodiesterase active site [InterPro reference PUB00007150].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
23 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| O34311 | Diguanylate cyclase DgcW Bacillus subtilis (strain 168) | 800 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR2 sibling references |
| O35014 | Uncharacterized EAL-domain containing protein YkuI Bacillus subtilis (strain 168) | 407 aa; version 1 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| P04162 | Transposon Tn21 modulator protein Escherichia coli | 116 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| P13518 | RNase E specificity factor CsrD Escherichia coli (strain K12) | 646 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR |
| P21514 | Cyclic di-GMP phosphodiesterase PdeL Escherichia coli (strain K12) | 362 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR1 sibling references |
| P23842 | Probable cyclic di-GMP phosphodiesterase PdeA Escherichia coli (strain K12) | 729 aa; version 2 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| P32701 | Probable cyclic di-GMP phosphodiesterase PdeC Escherichia coli (strain K12) | 528 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| P37646 | Cyclic di-GMP phosphodiesterase PdeH Escherichia coli (strain K12) | 255 aa; version 3 UniProt 2026_03 | PTM REGISTRY MIRROR |
| P37649 | Probable cyclic di-GMP phosphodiesterase PdeK Escherichia coli (strain K12) | 662 aa; version 3 UniProt 2026_03 | PTM REGISTRY MIRROR2 sibling references |
| P38097 | Probable diguanylate cyclase DgcE Escherichia coli (strain K12) | 1105 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR2 sibling references |
First 10 shown; the FASTA and family JSON contain all examples.
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 131,837
- matches
- 131,993
- proteomes
- 12,530
- taxa
- 17,849
- structures
- 75
- alphafold models
- 78,574
- domain architectures
- 3,876
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.