All families / PF00668
PF00668: Condensation
Condensation domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF00668: Condensation domain
IPR001242: integrated entry for Condensation domain
Source description
This domain is found in many multi-domain enzymes which synthesise peptide antibiotics. This domain catalyses a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (PF00550 (Pfam entry)). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site [InterPro reference PUB00007135].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
30 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| A0A0C1E5J8 | Nonribosomal peptide synthetase opaA Aspergillus ustus | 3901 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| A0A6G7BMN0 | Endopyrrole NRPS A Mycetohabitans rhizoxinica | 5199 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| A0ABS3A787 | Amino acid adenylation domain-containing protein Vibrio neptunius | 3992 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| A0ABS3A8C5 | Amino acid adenylation domain-containing protein Vibrio neptunius | 2049 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| A1DN09 | Nonribosomal peptide synthetase anaPS Aspergillus fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) | 2359 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| A6N339 | N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase Penicillium chrysogenum | 3791 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| B0XWK8 | Nonribosomal peptide synthetase hasD Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10) | 2273 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| B6F209 | Cyclo-acetoacetyl-L-tryptophan synthase Aspergillus oryzae | 3907 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| E2PZ83 | Phenyloxazoline synthase MbtB Streptomyces clavuligerus | 1096 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| G0REX6 | Hybrid PKS-NRPS synthetase iliA Trichoderma reesei (strain QM6a) | 3812 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
First 10 shown; the FASTA and family JSON contain all examples.
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 68,562
- matches
- 149,357
- proteomes
- 8,647
- taxa
- 14,069
- structures
- 107
- alphafold models
- 14,105
- domain architectures
- 7,481
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.