DUFMech

All families / PF00924

PF00924: MS_channel_2nd

Mechanosensitive ion channel, beta-domain

Curation status
SEEDED
Seed status
EX DUF
Characterization
UNSCORED

Grounding

PF00924: Mechanosensitive ion channel, beta-domain
IPR006685: integrated entry for Mechanosensitive ion channel, beta-domain

Source description

This entry includes small conductance mechanosensitive channels (MscS), which assembles to a homoheptameric complex [InterPro reference PUB00019883,InterPro reference PUB00019884,InterPro reference PUB00019885,InterPro reference PUB00101036,InterPro reference PUB00062568,InterPro reference PUB00101037]. Each monomer consists of a N-terminal transmembrane region and a large cytoplasmic segment composed of a five-stranded antiparallel beta-sheet (middle/second) domain and a C-terminal alpha/beta-domain [InterPro reference PUB00101036,InterPro reference PUB00062568,InterPro reference PUB00101037,InterPro reference PUB00064132]. This is the beta domain which adopts a Sm-like barrel structure.

Evidence and references

Source descriptions and links are provenance, not independent functional validation.

Evidence counts: not scored.

Curated functional assertions

No curated functional assertions supplied.

Protein sequence examples

22 verified sequence examples. FASTA

UniProtProtein / organismSequenceProvenance
F4IME1Mechanosensitive ion channel protein 7
Arabidopsis thaliana		849 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
5 sibling references
F4IME2Mechanosensitive ion channel protein 8
Arabidopsis thaliana		908 aa; version 2
UniProt 2026_03		UNIPROT FETCH
5 sibling references
O07594Uncharacterized MscS family protein YhdY
Bacillus subtilis (strain 168)		371 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
1 sibling references
O34897Uncharacterized MscS family protein YkuT
Bacillus subtilis (strain 168)		267 aa; version 2
UniProt 2026_03		UNIPROT FETCH
5 sibling references
O35043Uncharacterized MscS family protein YfkC
Bacillus subtilis (strain 168)		280 aa; version 1
UniProt 2026_03		UNIPROT FETCH
2 sibling references
P0AAT4Mechanosensing system component YbdG
Escherichia coli (strain K12)		415 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
8 sibling references
P0AEB5Low conductance mechanosensitive channel YnaI
Escherichia coli (strain K12)		343 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
2 sibling references
P0C0S1Small-conductance mechanosensitive channel
Escherichia coli (strain K12)		286 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
2 sibling references
P39285Miniconductance mechanosensitive channel MscM
Escherichia coli (strain K12)		1107 aa; version 3
UniProt 2026_03		PTM REGISTRY MIRROR
3 sibling references
P75783Moderate conductance mechanosensitive channel YbiO
Escherichia coli (strain K12)		741 aa; version 2
UniProt 2026_03		PTM REGISTRY MIRROR
2 sibling references

First 10 shown; the FASTA and family JSON contain all examples.

Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.

Sequences and metadata: UniProt Consortium, CC BY 4.0.

Domain matches

No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.

Snapshot counters

proteins
69,957
matches
69,995
proteomes
20,063
taxa
30,243
structures
65
alphafold models
46,372
domain architectures
274

Classification basis

pfam previous unknown name

No scoring reasons supplied.

Related Mech records

This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.

No cross-Mech examples are present in the supplied snapshot.

Curation history and reviews

No retained curation events supplied.

No retained review supplied.

Record source

data/families/PF00924.yaml
Family YAML in this build

Download this family (JSON)

Status and evidence

Seed status
A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
Characterization
A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
Evidence counts
Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
Curation status
Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
Missing counts
Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.