All families / PF01205
PF01205: Impact_N
Impact N-terminal domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF01205: Impact N-terminal domain
IPR001498: integrated entry for Impact N-terminal domain
Source description
This entry represents the N-terminal domain of the Impact proteins. The Impact protein is a translational regulator that ensures constant high levels of translation under amino acid starvation. It acts by interacting with Gcn1/Gcn1L1, thereby preventing activation of Gcn2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis [InterPro reference PUB00161305,InterPro reference PUB00158485]. It is evolutionary conserved from eukaryotes to archaea [InterPro reference PUB00046141].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
10 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| A7YY45 | Protein IMPACT Bos taurus | 318 aa; version 2 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| O55091 | Protein IMPACT Mus musculus | 318 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR9 sibling references
|
| P25637 | Protein IMPACT homolog Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 258 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR |
| P27862 | IMPACT family member YigZ Escherichia coli (strain K12) | 204 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR11 sibling references
|
| P32437 | IMPACT family member YvyE Bacillus subtilis (strain 168) | 217 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| P44842 | IMPACT family member HI_0722 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) | 206 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q12257 | IMPACT family member YDL177C Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 170 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q5GFD9 | Protein IMPACT Rattus norvegicus | 317 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q9P2X3 | Protein IMPACT Homo sapiens | 320 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q9XWF4 | Protein IMPACT homolog Caenorhabditis elegans | 257 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 18,592
- matches
- 18,629
- proteomes
- 16,361
- taxa
- 26,223
- structures
- 5
- alphafold models
- 12,878
- domain architectures
- 121
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.