DUFMech

All families / PF01909

PF01909: NTP_transf_2

Nucleotidyltransferase domain

Curation status
SEEDED
Seed status
EX DUF
Characterization
UNSCORED

Grounding

PF01909: Nucleotidyltransferase domain
IPR002934: integrated entry for Nucleotidyltransferase domain

Source description

Members of this family belong to a large family of nucleotidyltransferases [InterPro reference PUB00005745]. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.

Evidence and references

Source descriptions and links are provenance, not independent functional validation.

Evidence counts: not scored.

Curated functional assertions

No curated functional assertions supplied.

Protein sequence examples

31 verified sequence examples. FASTA

UniProtProtein / organismSequenceProvenance
E9Q9A92'-5'-oligoadenylate synthase 2
Mus musculus		742 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
O28126CCA-adding enzyme
Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)		437 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
P009732'-5'-oligoadenylate synthase 1
Homo sapiens		400 aa; version 4
UniProt 2026_03		PTM REGISTRY MIRROR
3 sibling references
P04053DNA nucleotidylexotransferase
Homo sapiens		509 aa; version 3
UniProt 2026_03		PTM REGISTRY MIRROR
5 sibling references
P0A0D1Spectinomycin 9-adenylyltransferase
Staphylococcus aureus (strain N315)		260 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
P0AG05Aminoglycoside (3'') (9) adenylyltransferase
Escherichia coli		263 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
P0DXA6Cyclic GMP-AMP synthase CdnE03
Staphylococcus schleiferi		324 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
P27249Bifunctional uridylyltransferase/uridylyl-removing enzyme
Escherichia coli (strain K12)		890 aa; version 2
UniProt 2026_03		PTM REGISTRY MIRROR
6 sibling references
P297282'-5'-oligoadenylate synthase 2
Homo sapiens		719 aa; version 3
UniProt 2026_03		UNIPROT FETCH
2 sibling references
P36223Bifunctional uridylyltransferase/uridylyl-removing enzyme
Azotobacter vinelandii		899 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references

First 10 shown; the FASTA and family JSON contain all examples.

Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.

Sequences and metadata: UniProt Consortium, CC BY 4.0.

Domain matches

No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.

Snapshot counters

proteins
24,660
matches
24,705
proteomes
12,011
taxa
19,472
structures
133
alphafold models
16,552
domain architectures
272

Classification basis

pfam previous unknown name

No scoring reasons supplied.

Related Mech records

This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.

No cross-Mech examples are present in the supplied snapshot.

Curation history and reviews

No retained curation events supplied.

No retained review supplied.

Record source

data/families/PF01909.yaml
Family YAML in this build

Download this family (JSON)

Status and evidence

Seed status
A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
Characterization
A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
Evidence counts
Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
Curation status
Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
Missing counts
Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.