All families / PF02996
PF02996: Prefoldin
Prefoldin subunit
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF02996: Prefoldin subunit
IPR004127: integrated entry for Prefoldin subunit
Source description
This family comprises of several prefoldin subunits. The biogenesis of the cytoskeletal proteins actin and tubulin involves interaction of nascent chains of each of the two proteins with the oligomeric protein prefoldin (PFD) and their subsequent transfer to the cytosolic chaperonin CCT (chaperonin containing TCP-1). Electron microscopy shows that eukaryotic PFD, which has a similar structure to its archaeal counterpart, interacts with unfolded actin along the tips of its projecting arms. In its PFD-bound state, actin seems to acquire a conformation similar to that adopted when it is bound to CCT [InterPro reference PUB00013187].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
20 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| O18054 | Probable prefoldin subunit 3 Caenorhabditis elegans | 185 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| O94763 | Unconventional prefoldin RPB5 interactor 1 Homo sapiens | 535 aa; version 3 UniProt 2026_03 | PTM REGISTRY MIRROR10 sibling references
|
| P43573 | Bud site selection protein 27 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 796 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| P48363 | Prefoldin subunit 3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 199 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| P57741 | Prefoldin subunit 3 Arabidopsis thaliana | 195 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR |
| P57742 | Prefoldin subunit 5 Arabidopsis thaliana | 151 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR14 sibling references
|
| P61758 | Prefoldin subunit 3 Homo sapiens | 197 aa; version 4 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| P61759 | Prefoldin subunit 3 Mus musculus | 196 aa; version 2 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q04493 | Prefoldin subunit 5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 163 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q21993 | Probable prefoldin subunit 5 Caenorhabditis elegans | 152 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
First 10 shown; the FASTA and family JSON contain all examples.
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 12,752
- matches
- 12,782
- proteomes
- 4,076
- taxa
- 10,385
- structures
- 9
- alphafold models
- 10,916
- domain architectures
- 219
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.