All families / PF04224
PF04224: DUF417
Protein of unknown function, DUF417
- Curation status
- SEEDED
- Seed status
- UNKNOWN CANDIDATE
- Characterization
- UNSCORED
Grounding
PF04224: Protein of unknown function, DUF417
IPR007339: integrated entry for Protein of unknown function, DUF417
Source description
This family of uncharacterised integral membrane proteins appears to be restricted to proteobacteria. These proteins are related to the DoxX family PF07681 (Pfam entry). Members of this family form a confident homodimeric structure, for example UniProtKB:A0A4R5FQM0 iPTM=0.94.
Evidence and references
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
3 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| P44577 | Uncharacterized protein HI_0219 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) | 213 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| P46928 | Uncharacterized 23.0 kDa protein in purT/mpa1 5'region Mannheimia haemolytica | 211 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| P75685 | Inner membrane protein RclC Escherichia coli (strain K12) | 197 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 1,971
- matches
- 2,090
- proteomes
- 1,536
- taxa
- 2,351
- structures
- 0
- alphafold models
- 1,271
- domain architectures
- 5
Classification basis
short name matches duf; name says unknown function
No scoring reasons supplied.
Related Mech records
| Family | Mech | Record | Protein | Link basis |
|---|---|---|---|---|
| DUF417 (PF04224) | ProteinTraitsMech | response to hypochlorite | P75685 Inner membrane protein RclC | canonical example family classification |
| DUF417 (PF04224) | ProteinTraitsMech | reactive chlorine resistance membrane protein RclC | P75685 Inner membrane protein RclC | canonical example family classification |
| DUF417 (PF04224) | ProteinTraitsMech | Protein of unknown function, DUF417 | P44577 Uncharacterized protein HI_0219 | canonical example family classification |
| DUF417 (PF04224) | ProteinTraitsMech | Protein of unknown function, DUF417 | P46928 Uncharacterized 23.0 kDa protein in purT/mpa1 5'region | canonical example family classification |
| DUF417 (PF04224) | ProteinTraitsMech | Protein of unknown function, DUF417 | P75685 Inner membrane protein RclC | canonical example family classification |
| DUF417 (PF04224) | ProteinTraitsMech | Protein of unknown function, DUF417 | trait identifier |
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.