All families / PF05891
PF05891: Methyltransf_PK
AdoMet dependent proline di-methyltransferase
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF05891: AdoMet dependent proline di-methyltransferase
IPR008576: integrated entry for AdoMet dependent proline di-methyltransferase
Source description
This protein is expressed in the tail neuron PVT and in uterine cells in C. elegans [worm-base]. In Saccharomyces cerevisiae this is AdoMet dependent proline di-methyltransferase. This enzyme catalyses the di-methylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues. The methyltransferases described here specifically recognise the N-terminal X-Pro-Lys sequence motif, and they may account for nearly all previously described eukaryotic protein N-terminal methylation reactions. A number of other yeast and human proteins also share the recognition motif and may be similarly modified [InterPro reference PUB00073462]. As with other methyltransferases, this family carries the characteristic GxGxG motif.
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
8 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| B2RXM4 | N-terminal Xaa-Pro-Lys N-methyltransferase 2 Mus musculus | 283 aa; version 1 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| P38340 | Alpha N-terminal protein methyltransferase 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 232 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q5PP70 | Alpha N-terminal protein methyltransferase 1 Arabidopsis thaliana | 276 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR |
| Q5VVY1 | N-terminal Xaa-Pro-Lys N-methyltransferase 2 Homo sapiens | 283 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q6NN40 | Alpha N-terminal protein methyltransferase 1 Drosophila melanogaster | 276 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q8R2U4 | N-terminal Xaa-Pro-Lys N-methyltransferase 1 Mus musculus | 223 aa; version 3 UniProt 2026_03 | UNIPROT FETCH |
| Q9BV86 | N-terminal Xaa-Pro-Lys N-methyltransferase 1 Homo sapiens | 223 aa; version 3 UniProt 2026_03 | UNIPROT FETCH9 sibling references |
| Q9N4D9 | Alpha N-terminal protein methyltransferase 1 Caenorhabditis elegans | 234 aa; version 2 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 5,051
- matches
- 5,426
- proteomes
- 3,284
- taxa
- 8,737
- structures
- 25
- alphafold models
- 4,329
- domain architectures
- 101
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.