All families / PF06664
PF06664: WLS-like_TM
Wntless-like, transmembrane domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF06664: Wntless-like, transmembrane domain
IPR047843: integrated entry for Wntless-like, transmembrane domain
Source description
This entry represents the seven transmembrane domain of wntless (WLS) from Drosophila and its homologues, also known as MIG-14 in C.elegans which regulates endocytosis and secretion of the wnt ligand egl-20[InterPro reference PUB00058252]. Newly synthesised EGL-20/Wnt binds to MIG-14 in the Golgi, targetting the Wnt to the cell membrane for secretion. AP-2-mediated endocytosis and retromer retrieval at the sorting endosome would recycle MIG-14 to the Golgi, where it can bind to EGL-20/Wnt for next cycle of secretion [InterPro reference PUB00058252,InterPro reference PUB00154330]. WLS is a membrane trafficking and secretion chaperone for lipidated Wnt signaling proteins [InterPro reference PUB00151072]. It regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism [InterPro reference PUB00151072,InterPro reference PUB00061859]. This entry also includes TMEM181, a cell surface protein associated with Escherichia coli CDT toxicity [InterPro reference PUB00151072]. Members of this entry are homologous to GPR180 and TMEM145, and together with TMEM87A/B, GPR107 and GPR108, they have been described as GOLD domain seven-transmembrane helix (GOST) proteins given their domain architecture similarities. They all consist of a GOLD domain atop of a seven-transmembrane helix domain [InterPro reference PUB00151072].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
5 verified sequence examples. FASTA
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 3,813
- matches
- 3,913
- proteomes
- 1,345
- taxa
- 4,379
- structures
- 9
- alphafold models
- 3,297
- domain architectures
- 54
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.