All families / PF20148
PF20148: DUF6531
Domain of unknown function (DUF6531)
- Curation status
- SEEDED
- Seed status
- UNKNOWN CANDIDATE
- Characterization
- UNSCORED
Grounding
PF20148: Domain of unknown function (DUF6531)
IPR045351: integrated entry for Domain of unknown function (DUF6531)
Source description
This putative domain is found in a range of RHS proteins.
Evidence and references
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
8 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| D4G3R4 | tRNA(Glu)-specific nuclease WapA Bacillus subtilis subsp. natto (strain BEST195) | 2347 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| P0DUH5 | Double-stranded DNA deaminase toxin A Burkholderia cenocepacia (strain H111) | 1427 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| P16916 | Protein RhsA Escherichia coli (strain K12) | 1377 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR2 sibling references |
| P16917 | Protein RhsB Escherichia coli (strain K12) | 1411 aa; version 4 UniProt 2026_03 | PTM REGISTRY MIRROR2 sibling references |
| P16918 | Protein RhsC Escherichia coli (strain K12) | 1397 aa; version 4 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| P16919 | Protein RhsD Escherichia coli (strain K12) | 1426 aa; version 3 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q07833 | tRNA nuclease WapA Bacillus subtilis (strain 168) | 2334 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q9I0F4 | Toxin protein Tse5 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1317 aa; version 1 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 8,183
- matches
- 8,191
- proteomes
- 4,011
- taxa
- 6,046
- structures
- 12
- alphafold models
- 994
- domain architectures
- 3,104
Classification basis
short name matches duf; name says unknown function; domain of unknown function
No scoring reasons supplied.
Related Mech records
| Family | Mech | Record | Protein | Link basis |
|---|---|---|---|---|
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function DUF6531 | trait identifier | |
| DUF6531 (PF20148) | ProteinTraitsMech | Carbohydrate binding domain | Q07833 tRNA nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Carbohydrate-binding module family 96 | D4G3R4 tRNA(Glu)-specific nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Carbohydrate-binding module family 96 | Q07833 tRNA nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF6531) | D4G3R4 tRNA(Glu)-specific nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF6531) | P0DUH5 Double-stranded DNA deaminase toxin A | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF6531) | Q07833 tRNA nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF6531) | trait identifier | |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF4329) | P16917 Protein RhsB | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | Domain of unknown function (DUF4329) | P16919 Protein RhsD | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | PAAR motif | P0DUH5 Double-stranded DNA deaminase toxin A | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS protein | P0DUH5 Double-stranded DNA deaminase toxin A | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS protein | P16918 Protein RhsC | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS protein | Q9I0F4 Toxin protein Tse5 | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS Repeat | D4G3R4 tRNA(Glu)-specific nuclease WapA | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS Repeat | P0DUH5 Double-stranded DNA deaminase toxin A | canonical example family classification |
| DUF6531 (PF20148) | ProteinTraitsMech | RHS Repeat | Q07833 tRNA nuclease WapA | canonical example family classification |
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.