DUFMech

All families / PF20148

PF20148: DUF6531

Domain of unknown function (DUF6531)

Curation status
SEEDED
Seed status
UNKNOWN CANDIDATE
Characterization
UNSCORED

Grounding

PF20148: Domain of unknown function (DUF6531)
IPR045351: integrated entry for Domain of unknown function (DUF6531)

Source description

This putative domain is found in a range of RHS proteins.

Evidence and references

Source descriptions and links are provenance, not independent functional validation.

Evidence counts: not scored.

Curated functional assertions

No curated functional assertions supplied.

Protein sequence examples

8 verified sequence examples. FASTA

UniProtProtein / organismSequenceProvenance
D4G3R4tRNA(Glu)-specific nuclease WapA
Bacillus subtilis subsp. natto (strain BEST195)
2347 aa; version 2
UniProt 2026_03
UNIPROT FETCH
3 sibling references
P0DUH5Double-stranded DNA deaminase toxin A
Burkholderia cenocepacia (strain H111)
1427 aa; version 1
UniProt 2026_03
UNIPROT FETCH
4 sibling references
P16916Protein RhsA
Escherichia coli (strain K12)
1377 aa; version 1
UniProt 2026_03
PTM REGISTRY MIRROR
2 sibling references
P16917Protein RhsB
Escherichia coli (strain K12)
1411 aa; version 4
UniProt 2026_03
PTM REGISTRY MIRROR
2 sibling references
P16918Protein RhsC
Escherichia coli (strain K12)
1397 aa; version 4
UniProt 2026_03
UNIPROT FETCH
1 sibling references
P16919Protein RhsD
Escherichia coli (strain K12)
1426 aa; version 3
UniProt 2026_03
UNIPROT FETCH
1 sibling references
Q07833tRNA nuclease WapA
Bacillus subtilis (strain 168)
2334 aa; version 2
UniProt 2026_03
UNIPROT FETCH
4 sibling references
Q9I0F4Toxin protein Tse5
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
1317 aa; version 1
UniProt 2026_03
UNIPROT FETCH
1 sibling references

Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.

Sequences and metadata: UniProt Consortium, CC BY 4.0.

Domain matches

No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.

Snapshot counters

proteins
8,183
matches
8,191
proteomes
4,011
taxa
6,046
structures
12
alphafold models
994
domain architectures
3,104

Classification basis

short name matches duf; name says unknown function; domain of unknown function

No scoring reasons supplied.

Related Mech records

FamilyMechRecordProteinLink basis
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function DUF6531
trait identifier
DUF6531 (PF20148)ProteinTraitsMechCarbohydrate binding domainQ07833
tRNA nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechCarbohydrate-binding module family 96D4G3R4
tRNA(Glu)-specific nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechCarbohydrate-binding module family 96Q07833
tRNA nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF6531)D4G3R4
tRNA(Glu)-specific nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF6531)P0DUH5
Double-stranded DNA deaminase toxin A
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF6531)Q07833
tRNA nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF6531)
trait identifier
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF4329)P16917
Protein RhsB
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechDomain of unknown function (DUF4329)P16919
Protein RhsD
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechPAAR motifP0DUH5
Double-stranded DNA deaminase toxin A
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS proteinP0DUH5
Double-stranded DNA deaminase toxin A
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS proteinP16918
Protein RhsC
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS proteinQ9I0F4
Toxin protein Tse5
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS RepeatD4G3R4
tRNA(Glu)-specific nuclease WapA
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS RepeatP0DUH5
Double-stranded DNA deaminase toxin A
canonical example family classification
DUF6531 (PF20148)ProteinTraitsMechRHS RepeatQ07833
tRNA nuclease WapA
canonical example family classification

Curation history and reviews

No retained curation events supplied.

No retained review supplied.

Record source

data/families/PF20148.yaml
Family YAML in this build

Download this family (JSON)

Status and evidence

Seed status
A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
Characterization
A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
Evidence counts
Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
Curation status
Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
Missing counts
Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.