indole test positive

METPO:1005011 · CLASS · SEEDED

A phenotype in which an organism tests positive in the indole test, indicating it produces indole from tryptophan.

Trait evidence (2)

DOI:10.1128/AEM.02787-15
Indole, a bacterial product of tryptophan degradation

Darkoh et al. support microbial indole production as a tryptophan-degradation phenotype and evaluate indole detection in bacterial cultures.
DOI:10.1128/AEM.02787-15
enterotoxigenic Escherichia coli strain H10407 produces 3.3 ± 0.22 mM indole during a 24-h period in the presence of 5 mM tryptophan

Darkoh et al. measured indole production by the enterotoxigenic E. coli H10407 strain under tryptophan-supplemented culture conditions.

Provenance

Identifier source: METPO (2026-06-12)
Definition source: DOI:10.1128/AEM.02787-15

Parent traits (1)

indole test METPO:1005010

Synonyms (1)

indole test RELATED_SYNONYM · metpo.owl

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

Escherichia coli ETEC H10407 NCBITaxon:316401 DOI:10.1128/AEM.02787-15 Enterotoxigenic E. coli H10407 produced millimolar indole after 24 h of growth with tryptophan supplementation.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve a non-assay parent for positive indole-test phenotypes before narrowing parent_traits below phenotype.

CURATION TODO RESOLVED indole-test-positive-assay-parent-gap · raised by codex · 2026-09-11

Not yet attached to a section of this record — a curator sets attaches_to (e.g. causal_graphs#some_edge) so the gap shows beside the mechanism it concerns.

METPO:1005010 is the seeded superclass of METPO:1005011, but METPO:1005010 defines an assay rather than a broader microbial trait class, so this record is temporarily parented directly to METPO:1000059 phenotype.

Resolution: METPO:1005010 is now represented as the seeded indole-test assay parent, so this assay-outcome phenotype can use its exact source superclass instead of a temporary direct phenotype parent; this resolves the exact METPO assay-parent gap, not the broader non-assay parent question for assay-outcome phenotypes.

Curation history

2026-09-11T06:46:27Z · SEEDED_FROM_METPO · seed_from_metpo
imported from data/raw/metpo.owl (CLASS)
2026-09-11T07:00:00Z · ENRICHED_FROM_LITERATURE · codex
Enriched the active METPO indole-test-positive phenotype with DOI-backed indole production evidence after a repository-wide duplicate review covering ignored and hidden files.
2026-09-12T21:38:12Z · RESOLVED_PARENT_GAP · codex
Reparented indole test positive below the newly curated METPO:1005010 indole test assay parent and marked the temporary assay-parent discussion resolved.