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Voges-Proskauer test positive

METPO:1005017 · CLASS · SEEDED

A phenotype in which an organism tests positive in the Voges-Proskauer test, indicating acetoin production.

Trait evidence (2)

  • https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf
    In the presence of KOH the intermediate acetoin is oxidized to diacetyl, a reaction which is catalyzed by a-naphthol (2). Diacetyl reacts with the guanidine group associated with molecules contributed by peptone in the medium, to form a pinkish-red-colored product (Fig. 2A).

    The ASM protocol PDF explains that the Voges-Proskauer reaction detects acetoin through Barritt chemistry that forms a pinkish-red product.

  • DOI:10.1128/JCM.42.8.3665-3669.2004
    In the ASM Manual of Clinical Microbiology, only 6 of the 18 biochemical tests used in the present investigation are listed for Klebsiella species: indole, MR, VP, LDC, ODC, and malonate

    Hansen et al. evaluated Voges-Proskauer as a conventional Klebsiella biochemical test listed in the ASM Manual of Clinical Microbiology.

Provenance

Identifier source
METPO (2026-06-12)
Definition source
https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf

Parent traits (1)

Synonyms (1)

  • voges-proskauer test RELATED_SYNONYM · metpo.owl

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve a non-assay parent for positive Voges-Proskauer-test phenotypes before narrowing parent_traits below phenotype.

CURATION TODO RESOLVED voges-proskauer-test-positive-assay-parent-gap · raised by codex · 2026-09-12

Not yet attached to a section of this record — a curator sets attaches_to (e.g. causal_graphs#some_edge) so the gap shows beside the mechanism it concerns.

METPO:1005016 is the seeded superclass of METPO:1005017, but METPO:1005016 defines an assay rather than a broader microbial trait class, so this record is temporarily parented directly to METPO:1000059 phenotype.

Resolution: METPO:1005016 is now represented as the seeded Voges-Proskauer-test assay parent, so this positive assay-outcome phenotype can use its exact source superclass instead of a temporary direct phenotype parent; this resolves the exact METPO assay-parent gap, not the broader non-assay parent question for assay-outcome phenotypes.

Curation history

  1. · SEEDED_FROM_METPO · seed_from_metpo

    imported from data/raw/metpo.owl (CLASS)

  2. · ENRICHED_FROM_LITERATURE · codex

    Added the METPO Voges-Proskauer-test-positive phenotype with stable-URL and DOI-backed acetoin-production evidence after a repository-wide duplicate review covering ignored and hidden files.

  3. · RESOLVED_PARENT_GAP · codex

    Reparented Voges-Proskauer test positive below the newly curated METPO:1005016 Voges-Proskauer test assay parent and marked the temporary assay-parent discussion resolved.