primary homothallism

Trait evidence (4)

• DOI:10.1371/journal.pgen.1006110

  co-occurrence in the genome of a single individual of all the MAT alleles required to trigger sexual development

  David-Palma et al. (2016), PMID:27327578, PMC4915694. Exact Introduction sec001 clause defining primary homothallism, not an abstract quote. Abstract sections, Introduction, all Results and Methods sec011/sec014/sec015 were read. Actual Figures 1, 3 and 4, Table 1 and supplementary Table S3 were inspected. Methods/tables/images identify CBS 6938; prose also says CBS 6838, a retained source inconsistency. Single receptor deletions retain sporulation; double deletion blocks it and rescue restores it. HD2 deletion retains vestigial sporulation; an obligatory HD1/HD2 heterodimer is not established. Figure 3's caption swaps C/D: the actual C is the count plot. Table S3's Figure 2 E/G pointers correspond to actual Figure 1 E/F. SPO11-mutant basidia counts are not significantly reduced; spore viability is. Other figures, supplements and unread Methods/Discussion remain uninspected. Functional genetics supports the model, not MAT sequence presence alone.

• DOI:10.5598/imafungus.2015.06.01.13

  combined expression of MAT1-1 and MAT1-2 genes in a single genome

  2015 terminology review, PMID:26203424, PMC4500084. Exact contiguous phrase from section s2a Primary homothallism, read end-to-end in full-text XML. This is terminology authority, not experimental replication. Its MAT1-1/MAT1-2 formulation describes the ascomycete framework; do not impose that nomenclature on basidiomycetes. The section permits linked or unlinked loci and distinguishes other routes to self-fertility. Its Aspergillus example does not require eight uninucleate spores in every primary-homothallic fungus. Actual Figure 1 remains uninspected.

• DOI:10.1016/j.cub.2007.07.012

  self-fertilization does not bypass requirements for outcrossing sex but instead requires activation of these pathways within a single individual.

  Paoletti et al. (2007), PMID:17669651. Exact contiguous clause from the directly retrieved Europe PMC scientific abstract. In Aspergillus nidulans, alpha- and HMG-domain MAT gene expression is required for normal sexual development and ascospore production; balanced overexpression and an mpkB mutant test distinct perturbations. This does not establish universal dependence on every MAT gene in other fungi. Full text, methods, figures, supplements and original strain provenance remain uninspected; no canonical example is inferred.

• DOI:10.1128/EC.00019-10

  produced the same number of perithecia as the WT, containing mature, viable ascospores at day 7 of development.

  Klix et al. (2010), PMID:20435701, PMC2901639. Exact first Results subsection clause, whose subjects are the SmtA-1 and SmtA-3 deletion strains. Author-institution published PDF at https://www.uni-goettingen.de/de/document/download/a343d8aa3ca6ea7528fe002c2281f0fb.pdf/60.pdf was read after Europe PMC full-text failure; NCBI efetch returned front matter only. Abstract, Introduction, first two Results subsections, strain/culture and microscopy Methods were read; actual Figures 1/2 were inspected. On SWG at 24 C, SmtA-2 deletion instead arrests development at protoperithecia; rescue restores fertility. Thus all MAT genes are not individually essential. The abstract calls SmtA-3 an alpha-domain protein, whereas Introduction/Figure 1 report no known functional domain; do not infer an HMG domain from its MAT1-1-3 relationship. Other experiments, Discussion, supplements and natural provenance of S48977 remain unverified.