proteaphagy

Trait evidence (3)

• DOI:10.1074/jbc.M115.699124

  Here we show that in yeast, upon nitrogen starvation, proteasomes are targeted for vacuolar degradation through autophagy.

  PMID:26670610, PMC4751371. Scientific abstract directly read in DOI-matched Europe PMC metadata and XML. Yeast-strain and immunoblot Methods, autophagy-dependence and core/regulatory-particle Results, Table 1 and relevant captions were read. Saccharomyces cerevisiae reporter processing and vacuolar localization support degradation; native gels address incorporation into complexes. The core and regulatory particles can be targeted separately, with different Ubp3 dependence; intact 26S uptake is not required by the definition. Figure 6D's caption conflicts with its title and body concerning Rpn10, so no Rpn10 requirement is curated. Actual figures, supplements and independent natural-strain provenance were not inspected.

• DOI:10.1016/j.celrep.2016.07.015

  Here, we define two proteaphagy routes in yeast that respond to either nitrogen starvation or particle inactivation.

  PMID:27477278. Scientific abstract directly read in Europe PMC with matching DOI. The yeast study distinguishes starvation from quality-control turnover of inactive proteasomes; Cue5 and Hsp42 support the latter route, not a universal microbial gene inventory. Correction DOI:10.1016/j.celrep.2022.110552 (PMID:35294886) is linked by the source metadata. Its text was directly read at https://profiles.wustl.edu/en/publications/erratum-autophagic-turnover-of-inactive-26s-proteasomes-in-yeast-/: a duplicated anti-histone H3 control blot in Figure S3 was replaced; the authors state their conclusions are unchanged. The corrected figure, original full Methods, actual figures and strain provenance were not inspected. The correction is not independent trait evidence.

• DOI:10.1016/j.jbc.2021.101494

  Indeed, we found that several conditions that activated general autophagy did not induce proteaphagy, further distinguishing proteaphagy from general autophagy.

  PMID:34919962, PMC8732087. Scientific abstract directly read in DOI-matched Europe PMC metadata and XML; Results sec1.1-sec1.3, Discussion sec2 and Methods sec3.1-sec3.4 were read. Proteasome GFP processing, native gels and vacuolar microscopy distinguish cargo turnover from general autophagy. In sec1.3, ATG11 deletion abolishes residual nitrogen-starvation turnover in an ATG17 deletion background, not all proteaphagy in every condition. Rapamycin yields no detected difference in the ATG11 single deletion, with a stated detection-limit caveat. Thus the later result does not establish a blanket contradiction of the 2016 single-deletion result. Methods identify W303-derived SUB61/SUB62 reporter and deletion strains. Captions were read; actual figures, supplements and independent strain provenance were not inspected.