eisosome filament

GO:0036286 · Cytoskeleton · Filament · Proposed

A filamentous cortical structure formed, in S. pombe, by the eisosome component Pil1. [GO:0036286]

Part of: eisosome (GO:0032126); GO:0030863 — cortical cytoskeleton (no local record)

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Pil1/Lsp1 BAR-domain eisosome core	Protein	part of	`InterPro:IPR028245`	PIL1, LSP1			Fungal BAR-domain proteins that assemble into the cytoplasmic filament core of the eisosome. `DOI:10.1091/mbc.e10-12-1021` Olivera-Couto et al. 2011 identified Pil1 and Lsp1 as the BAR-domain core components of Saccharomyces cerevisiae eisosomes. `DOI:10.1083/jcb.201104040` Karotki et al. 2011 reconstituted purified yeast Pil1 and Lsp1 into filaments and membrane-associated scaffolds.

NCBITaxon:4751 Fungi — VARIABLE: BAR-domain eisosome filament scaffolds have been described in fungal MCC/eisosome domains, but the component set and domain architecture vary by lineage. [PMID:25002088]
NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding-yeast Pil1 and Lsp1 form the BAR-domain filament core of eisosomes. [DOI:10.1091/mbc.e10-12-1021]

NCBITaxon:4932 Saccharomyces cerevisiae — Budding-yeast model in which Pil1/Lsp1 BAR-domain proteins were reconstituted into eisosome-like membrane scaffolds. [DOI:10.1083/jcb.201104040]

MCC scaffold assembly — Pil1/Lsp1 eisosome filaments assemble along the cytoplasmic face of MCC membrane domains to help scaffold stable plasma-membrane furrows.
- DOI:10.1083/jcb.201104040 Karotki et al. 2011 connected Pil1/Lsp1 filament assembly to a membrane scaffold at eisosomes.
- PMID:25002088 Douglas and Konopka 2014 reviewed MCC/eisosomes as stable plasma-membrane domains in fungi.

Pil1 and Lsp1 assemble into a cytoplasmic eisosome filament that scaffolds the paired MCC plasma-membrane domain.

Subject	Predicate	Object	Evidence
Pil1/Lsp1 BAR-domain eisosome core	assembles into	eisosome filament	`DOI:10.1091/mbc.e10-12-1021` Olivera-Couto et al. 2011 established Pil1 and Lsp1 as the BAR-domain core components of Saccharomyces cerevisiae eisosomes.
eisosome filament	scaffolds	eisosome membrane domain/MCC	`DOI:10.1083/jcb.201104040` Karotki et al. 2011 showed that purified Pil1 and Lsp1 assemble into eisosome-like membrane scaffolds.

GO:0036286 Gene Ontology cellular-component term for eisosome filament.
GO:0032991 Gene Ontology cellular-component parent term for protein-containing complex.
GO:0032126 Gene Ontology cellular-component term for eisosome.
GO:0030863 — cortical cytoskeleton Gene Ontology cellular-component term for cortical cytoskeleton.
GO:0090512 Gene Ontology cellular-component term for eisosome membrane domain/MCC.
DOI:10.1091/mbc.e10-12-1021 Olivera-Couto et al. 2011, The eisosome core is composed of BAR domain proteins, Molecular Biology of the Cell.
DOI:10.1083/jcb.201104040 Karotki et al. 2011, Eisosome proteins assemble into a membrane scaffold, Journal of Cell Biology.
PMID:25002088 Douglas and Konopka 2014, Fungal membrane organization: the eisosome concept, Annual Review of Microbiology.

2026-09-15T22:55:13Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-15T22:55:52Z codex · EXPANDED_RECORD — Added protein-complex parentage, eisosome and cortical-cytoskeleton parthood, the InterPro-grounded Pil1/Lsp1 BAR-domain component, fungal and Saccharomyces scope, MCC scaffold assembly evidence, and a Pil1/Lsp1 filament assembly graph.
2026-09-15T23:06:38Z codex · ADDRESS_REVIEW — Resolved #1037 by removing unsupported ESSENTIAL from the aggregate Pil1/Lsp1 BAR-domain component.
2026-09-21T12:05:18Z codex · REPLACED_UNREADABLE_EVIDENCE — Resolved #1239 by routing Douglas and Konopka 2014 fungal eisosome-filament evidence through reachable PMID:25002088 instead of the unreadable Annual Reviews DOI.