autophagosome lumen

GO:0034423 · Membrane organelle · Subcellular region · Proposed

The volume enclosed within the autophagosome double-membrane. [GO:0034423]

Synonyms: Autophagic vacuolar lumen; Autophagic vacuole lumen; Autophagosomal lumen

Part of: autophagosome (GO:0005776)

Xrefs: uniprot.location:SL-0318 — Autophagosome lumen

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast forms double-membrane autophagosomes during starvation-induced macroautophagy. [DOI:10.1083/jcb.119.2.301]

NCBITaxon:4932 Saccharomyces cerevisiae — Genetic and fluorescence-microscopy model for the pre-autophagosomal structure and autophagosome formation. [DOI:10.1093/emboj/20.21.5971]

The autophagosome membrane bounds the autophagosome lumen inside the double-membrane autophagosome.

Subject	Predicate	Object	Evidence
autophagosome lumen	is part of	autophagosome	`GO:0034423` GO:0034423 identifies the autophagosome lumen. `GO:0005776` GO:0005776 identifies the enclosing autophagosome.
autophagosome membrane	bounds	autophagosome lumen	`GO:0034423` GO:0034423 defines the autophagosome lumen as the volume enclosed within the autophagosome double-membrane. `GO:0000421` GO:0000421 identifies the surrounding autophagosome membrane.

GO:0034423 Gene Ontology cellular-component term for the autophagosome lumen.
GO:0060205 Gene Ontology cellular-component parent term for cytoplasmic vesicle lumen.
GO:0005776 Gene Ontology cellular-component term for the enclosing autophagosome.
GO:0000421 Gene Ontology cellular-component term for the autophagosome membrane bounding the lumen.
uniprot.location:SL-0318 — Autophagosome lumen UniProt Subcellular Location entry SL-0318 maps to GO:0034423, places autophagosome lumen under cytoplasmic vesicle lumen, and names it part of autophagosome.
DOI:10.1083/jcb.119.2.301 Takeshige et al. 1992, Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction, Journal of Cell Biology.
DOI:10.1093/emboj/20.21.5971 Suzuki et al. 2001, The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation, The EMBO Journal.

2026-09-14T22:54:05Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-14T22:54:32Z codex · EXPANDED_RECORD — Added UniProt synonyms, cytoplasmic-vesicle-lumen parentage, autophagosome parthood, the UniProt SL-0318 xref, Saccharomyces scope, GO/UniProt/DOI evidence, and a nonmechanistic autophagosome-lumen topology graph.