autophagosome membrane

GO:0000421 · Membrane organelle · Membrane · Proposed

The membrane surrounding the autophagosome. [GO:0000421]

Synonyms: Autophagic vacuolar membrane; Autophagic vacuole membrane; Autophagosomal membrane

Part of: autophagosome (GO:0005776)

Xrefs: uniprot.location:SL-0022 — Autophagosome membrane

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
autophagosome lipid bilayer	Lipid	part of	Reviewed label only (reviewed name; no accession asserted)			ESSENTIAL	Lipid-bilayer material that forms the autophagosome membrane boundary enclosing cytoplasmic cargo during macroautophagy. `GO:0000421` GO:0000421 identifies the autophagosome membrane surrounding an autophagosome. `uniprot.location:SL-0022` — Autophagosome membrane UniProt SL-0022 maps directly to GO:0000421 and describes the membrane surrounding an autophagosome. `DOI:10.1038/ncb1007-1102` Xie and Klionsky 2007 review autophagosome biogenesis and the membrane-expansion machinery that forms the compartment.

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast forms autophagosomes during starvation-induced macroautophagy. [DOI:10.1083/jcb.119.2.301]

NCBITaxon:4932 Saccharomyces cerevisiae — Genetic and fluorescence-microscopy model for the pre-autophagosomal structure and autophagosome formation. [DOI:10.1093/emboj/20.21.5971]

macroautophagy GO:0016236 — The autophagosome membrane expands and closes around cytoplasmic cargo to produce the double-membrane carrier used in macroautophagy.
- GO:0016236 GO:0016236 defines macroautophagy as the autophagic process that proceeds via autophagosome formation.
- DOI:10.1038/ncb1007-1102 Xie and Klionsky 2007 review the core autophagosome membrane-formation machinery.

The autophagosome membrane is part of the autophagosome and bounds the autophagosome lumen during macroautophagy.

Subject	Predicate	Object	Evidence
autophagosome membrane	bounds	autophagosome lumen	`GO:0000421` GO:0000421 identifies the autophagosome membrane. `GO:0034423` GO:0034423 defines the autophagosome lumen as the volume enclosed within the autophagosome double-membrane.
autophagosome membrane	is part of	autophagosome	`GO:0000421` GO:0000421 identifies the autophagosome membrane. `uniprot.location:SL-0022` — Autophagosome membrane UniProt SL-0022 names autophagosome as the parent compartment.
autophagosome membrane	supports	macroautophagy	`GO:0016236` GO:0016236 defines macroautophagy as proceeding via autophagosome formation. `DOI:10.1038/ncb1007-1102` Xie and Klionsky 2007 review autophagosome membrane formation.

CURATION_TODO (OPEN): Resolve an exact source-neutral grounding for the lipid constituent of the autophagosome membrane. The record is exactly grounded to GO:0000421 and UniProt SL-0022, but no exact source-neutral CURIE was curated for the generic lipid constituent of the autophagosome membrane.

GO:0000421 Gene Ontology cellular-component term for the autophagosome membrane.
GO:0030659 Gene Ontology cellular-component parent term for cytoplasmic vesicle membrane.
GO:0005776 Gene Ontology cellular-component term for the enclosing autophagosome.
GO:0034423 Gene Ontology cellular-component term for the lumen bounded by the autophagosome membrane.
GO:0016236 Gene Ontology biological-process term for macroautophagy.
uniprot.location:SL-0022 — Autophagosome membrane UniProt Subcellular Location entry SL-0022 maps to GO:0000421, places autophagosome membrane under cytoplasmic vesicle membrane, and names autophagosome as the parent compartment.
DOI:10.1083/jcb.119.2.301 Takeshige et al. 1992, Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction, Journal of Cell Biology.
DOI:10.1093/emboj/20.21.5971 Suzuki et al. 2001, The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation, The EMBO Journal.
DOI:10.1038/ncb1007-1102 Xie and Klionsky 2007, Autophagosome formation: core machinery and adaptations, Nature Cell Biology.

2026-09-14T23:11:20Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-14T23:12:02Z codex · EXPANDED_RECORD — Added UniProt synonyms, cytoplasmic-vesicle membrane parentage, autophagosome parthood, the UniProt SL-0022 xref, a reviewed-label-only lipid-bilayer component, budding-yeast scope, GO/UniProt/DOI evidence, a macroautophagy function, and a nonmechanistic autophagosome-membrane topology graph.