glyoxysomal lumen

GO:0031908 · Membrane organelle · Subcellular region · Proposed

The volume enclosed by the glyoxysome membrane. [GO:0031908]

Synonyms: glyoxysomal matrix; glyoxysome lumen; glyoxysome matrix

Part of: glyoxysome (GO:0009514)

Xrefs: uniprot.location:SL-0333 — Glyoxysome matrix

Taxonomic distribution

NCBITaxon:5141 Neurospora crassa — COMMON: Neurospora crassa forms acetate-induced glyoxysomes with luminal matrix enzymes targeted through C-terminal glyoxysomal targeting signals. [DOI:10.1007/BF00309930]

NCBITaxon:5141 Neurospora crassa — Filamentous-fungal model used to test import of matrix proteins into the glyoxysomal lumen under acetate-induced growth conditions. [DOI:10.1007/BF00309930]

glyoxylate cycle GO:0006097 — The glyoxysomal lumen is the matrix compartment that contains glyoxylate-cycle enzymes.
- GO:0009514 GO:0009514 defines glyoxysomes as peroxisome specializations that contain glyoxylate-pathway enzymes.
- DOI:10.1007/BF00309930 de Zoysa and Connerton 1994 studied targeting of matrix enzymes into Neurospora crassa glyoxysomes.

The glyoxysomal lumen is the internal glyoxysome compartment enclosed by the glyoxysomal membrane.

Subject	Predicate	Object	Evidence
glyoxysomal lumen	is part of	glyoxysome	`GO:0031908` GO:0031908 identifies the glyoxysomal lumen cellular component. `uniprot.location:SL-0333` — Glyoxysome matrix UniProt SL-0333 places the glyoxysome matrix under the glyoxysome subcellular location. `GO:0009514` GO:0009514 identifies the containing glyoxysome.
glyoxysomal membrane	encloses	glyoxysomal lumen	`GO:0031908` GO:0031908 defines the glyoxysomal lumen as the volume enclosed by the glyoxysome membrane. `GO:0046861` GO:0046861 identifies the membrane surrounding a glyoxysome. `uniprot.location:SL-0130` — Glyoxysome membrane UniProt SL-0130 maps the glyoxysome membrane to GO:0046861.
glyoxylate cycle	occurs in	glyoxysomal lumen	`GO:0009514` GO:0009514 defines glyoxysomes by their glyoxylate-pathway enzyme content. `DOI:10.1007/BF00309930` de Zoysa and Connerton 1994 studied targeting of glyoxylate-cycle enzymes into the Neurospora crassa glyoxysomal matrix.

INTERPRETATION (RESOLVED): Keep the glyoxysomal membrane as the boundary of the glyoxysomal lumen rather than as a lumen component. GO:0031908 denotes the volume enclosed by the glyoxysome membrane, whereas GO:0046861 denotes the membrane surrounding a glyoxysome.

GO:0031908 Gene Ontology cellular-component term for glyoxysomal lumen.
GO:0043233 Gene Ontology cellular-component term for organelle lumen, the broader organelle-enclosed compartment parent.
GO:0009514 Gene Ontology cellular-component term for the containing glyoxysome.
GO:0046861 Gene Ontology cellular-component term for the glyoxysomal membrane that encloses the lumen.
GO:0006097 Gene Ontology biological-process term for the glyoxylate cycle.
uniprot.location:SL-0333 — Glyoxysome matrix UniProt Subcellular Location entry SL-0333 maps Glyoxysome matrix to GO:0031908, places it under Glyoxysome, and lists glyoxysomal lumen, glyoxysomal matrix, and glyoxysome lumen as exact synonyms.
uniprot.location:SL-0130 — Glyoxysome membrane UniProt Subcellular Location entry SL-0130 maps Glyoxysome membrane to GO:0046861.
DOI:10.1007/BF00309930 de Zoysa and Connerton 1994, The function and specificity of the C-terminal tripeptide glyoxysomal targeting signal in Neurospora crassa, Current Genetics.

2026-09-13T16:38:53Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-13T16:39:26Z codex · EXPANDED_RECORD — Added glyoxysome matrix synonyms and the UniProt SL xref, organelle-lumen parentage, glyoxysome parthood, Neurospora scope, a glyoxylate-cycle function, GO/UniProt/DOI evidence, a nonmechanistic lumen-topology graph, and a resolved boundary interpretation.
2026-09-13T16:43:21Z codex · ADDRESS_VALIDATION — Normalized the GO:0006097 glyoxylate-cycle function label to the authority label required by id-label correspondence validation.