multivesicular body intraluminal vesicle

cellstructuremech:multivesicular_body_intraluminal_vesicle · Membrane organelle · Membrane bounded organelle · Proposed

A membrane-bounded vesicle inside a multivesicular body that forms when the limiting endosomal membrane buds inward and pinches into the lumen during multivesicular body sorting. [DOI:10.1016/j.devcel.2011.05.015]

Part of: multivesicular body (GO:0005771)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
intraluminal vesicle lipid bilayer	Lipid	part of	Reviewed label only (reviewed name; no accession asserted)			ESSENTIAL	Lipid-bilayer constituent that buds from the multivesicular-body limiting membrane to enclose each intraluminal vesicle. `GO:0005771` GO:0005771 defines multivesicular bodies as endosomes with internal vesicles formed by invagination of the limiting endosomal membrane. `DOI:10.1016/j.devcel.2011.05.015` Henne, Buchkovich and Emr 2011 reviewed intraluminal vesicle formation as inward budding of the endosomal limiting membrane in the ESCRT pathway.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast forms multivesicular-body intraluminal vesicles during ESCRT-dependent sorting of ubiquitinated membrane cargo. [PMID:11511343]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Genetic model for ESCRT-dependent sorting at endosomal membranes and formation of intraluminal vesicles in multivesicular bodies. [PMID:12194857]

Functions

multivesicular body sorting pathway GO:0071985 — Multivesicular-body intraluminal vesicles receive ubiquitinated transmembrane cargo from the limiting endosomal membrane and sequester it inside the multivesicular-body lumen.
- GO:0071985 GO:0071985 denotes the multivesicular body sorting pathway that targets ubiquitylated transmembrane proteins into luminal vesicles.
- PMID:11511343 Katzmann, Babst and Emr 2001 identified ESCRT-I as a conserved yeast endosomal protein-sorting complex needed for ubiquitin-dependent MVB cargo sorting.
- DOI:10.1016/j.devcel.2011.05.015 Henne, Buchkovich and Emr 2011 reviewed the ESCRT pathway as a route that sorts endosomal cargo into intraluminal vesicles for lysosomal or vacuolar degradation.

Mechanism graphs

ESCRT machinery forms multivesicular-body intraluminal vesicles (ASSEMBLY)

In Saccharomyces cerevisiae, ESCRT-III remodels the multivesicular-body limiting membrane so it buds inward and forms intraluminal vesicles in the multivesicular-body lumen.

Subject	Predicate	Object	Evidence
ESCRT III complex	remodels	multivesicular body membrane	`PMID:12194857` Babst et al. 2002 characterized yeast ESCRT-III as an endosome-associated complex required for MVB sorting. `DOI:10.1016/j.devcel.2011.05.015` Henne, Buchkovich and Emr 2011 reviewed ESCRT-III as the membrane-remodeling complex in the endosomal ESCRT pathway.
multivesicular body membrane	buds inward to form	multivesicular body intraluminal vesicle	`GO:0005771` GO:0005771 defines multivesicular bodies as endosomes with internal vesicles formed by invagination of the limiting endosomal membrane.
multivesicular body intraluminal vesicle	is contained in	multivesicular body lumen	`GO:0005771` GO:0005771 defines the multivesicular body by the presence of internal vesicles. `uniprot.location:SL-0340` — Multivesicular body lumen UniProt SL-0340 identifies the internal multivesicular body lumen.

Discussions

CURATION_TODO (OPEN): Resolve an exact source-neutral grounding for the lipid bilayer of multivesicular-body intraluminal vesicles. The record is grounded as an intraluminal-vesicle substructure of the multivesicular body, but this pass did not curate a separate CURIE for the membrane lipid constituent of one vesicle.
INTERPRETATION (RESOLVED): Keep ESCRT complexes and cargo-recognition factors out of the multivesicular-body intraluminal vesicle component list. ESCRT machinery sorts cargo and drives limiting-membrane remodeling during intraluminal-vesicle formation, but it is modeled as assembly machinery rather than as a stable molecular constituent of the resulting vesicle.

Evidence

GO:0031982 Gene Ontology cellular-component term for the broader vesicle class.
GO:0005771 Gene Ontology cellular-component term for multivesicular bodies, defined by internal vesicles formed by limiting-membrane invagination.
GO:0032585 Gene Ontology cellular-component term for the multivesicular body membrane that buds inward to generate intraluminal vesicles.
GO:0071985 Gene Ontology biological-process term for the multivesicular body sorting pathway.
uniprot.location:SL-0340 — Multivesicular body lumen UniProt Subcellular Location entry SL-0340 identifies the multivesicular body lumen as the compartment bounded by the multivesicular body membrane.
PMID:11511343 Katzmann, Babst and Emr 2001, Ubiquitin-Dependent Sorting into the Multivesicular Body Pathway Requires the Function of a Conserved Endosomal Protein Sorting Complex, ESCRT-I, Cell.
PMID:12194857 Babst, Katzmann, Estepa-Sabal, Meerloo and Emr 2002, ESCRT-III, Developmental Cell.
DOI:10.1016/j.devcel.2011.05.015 Henne, Buchkovich and Emr 2011, The ESCRT Pathway, Developmental Cell.

Curation history

2026-09-23T08:12:51Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-23T08:14:15Z codex · EXPANDED_RECORD — Added vesicle parentage, multivesicular-body parthood, a reviewed-label-only lipid-bilayer component, budding-yeast scope, GO/PMID/DOI evidence, a nonmechanistic ESCRT intraluminal-vesicle formation graph, and lipid and ESCRT component-boundary discussions.
2026-09-23T08:26:33Z codex · ADDRESS_REVIEW — Resolved #1372 by adding UniProt SL-0340 evidence to the MVB-lumen containment edge and resolved #1373 by removing the over-specific completed-vesicle cargo containment edge from the ILV assembly graph.

Source: data/structures/membrane_organelle/multivesicular_body_intraluminal_vesicle.yaml