vacuolar lumen

GO:0005775 · Membrane organelle · Subcellular region · Proposed

The volume enclosed within the vacuolar membrane. [GO:0005775]

Part of: vacuole (GO:0005773)

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast has an acidic lumen inside its lysosome-like vacuole. [DOI:10.1016/j.bbamcr.2008.08.003]

NCBITaxon:4932 Saccharomyces cerevisiae — Budding yeast has an acidic fungal-type vacuole lumen inside its lysosome-like vacuole. [DOI:10.1016/j.bbamcr.2008.08.003]

vacuolar lumen compartmentalization — The vacuolar lumen is the membrane-enclosed compartment in which vacuolar contents and soluble activities are isolated from the cytosol.
- GO:0005775 GO:0005775 defines the vacuolar lumen as the volume enclosed by the vacuolar membrane.
- DOI:10.1016/j.bbamcr.2008.08.003 Li and Kane 2009 review the membrane-enclosed yeast vacuole lumen as the site of lysosome-like storage and degradation.

The vacuolar lumen is the internal vacuolar compartment bounded by the vacuolar membrane.

Subject	Predicate	Object	Evidence
vacuolar lumen	is part of	vacuole	`GO:0005775` GO:0005775 identifies the vacuolar lumen and places it inside the vacuole. `GO:0005773` GO:0005773 identifies the containing vacuole.
vacuolar membrane	bounds	vacuolar lumen	`GO:0005775` GO:0005775 defines the lumen as the volume enclosed within the vacuolar membrane. `GO:0005774` GO:0005774 identifies the bounding vacuolar membrane.

INTERPRETATION (RESOLVED): Keep the vacuolar membrane as the boundary of the vacuolar lumen rather than as a lumen component. GO:0005775 defines the vacuolar lumen by the surrounding vacuolar membrane, so the membrane is represented as a boundary node and not as a molecular component of the lumen.

GO:0005775 Gene Ontology cellular-component term for the vacuolar lumen.
GO:0070013 Gene Ontology cellular-component parent term for intracellular organelle lumen.
GO:0005773 Gene Ontology cellular-component term for the vacuole that contains the vacuolar lumen.
GO:0005774 Gene Ontology cellular-component term for the vacuolar membrane that bounds the lumen.
DOI:10.1016/j.bbamcr.2008.08.003 Li and Kane 2009, The yeast lysosome-like vacuole: Endpoint and crossroads, Biochimica et Biophysica Acta.

2026-09-15T09:30:21Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-15T09:31:21Z codex · EXPANDED_RECORD — Added organelle-lumen parentage, vacuole parthood, Eukaryota scope, a Saccharomyces example, lumen compartmentalization, GO/DOI evidence, a nonmechanistic membrane/lumen topology graph, and a resolved lumen-boundary interpretation.
2026-09-15T09:42:48Z codex · REVIEW_FIX — Addressed PR #957 review issues #958, #959, and #960 by narrowing distribution to Saccharomyces evidence, retyping the lumen and vacuole graph nodes, and marking the topology graph as FUNCTION.