CCR4-NOT core complex

GO:0030015 · Other · Multiprotein complex · Proposed

The core of the CCR4-NOT complex. In Saccharomyces the CCR4-NOT core complex comprises Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p, and Not5p. [GO:0030015]

Synonyms: 1.0 MDa CCR4-NOT complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Ccr4 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	CCR4	UNKNOWN	Poly(A)-specific ribonuclease constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf1/Pop2 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	CAF1, POP2	UNKNOWN	Caf1/Pop2 ribonuclease-family constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not1/Cdc39 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NOT1, CDC39	UNKNOWN	Large protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not2/Cdc36 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NOT2, CDC36	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not3 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NOT3	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not4/Mot2 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NOT4, MOT2	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not5 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NOT5	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf40 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	CAF40	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf130 CCR4-NOT core subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	CAF130	UNKNOWN	Protein constituent of the budding-yeast CCR4-NOT core complex. `GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which GO:0030015 defines the nine-subunit CCR4-NOT core complex and Chen et al. 2001 purified the 1.0 MDa complex. [GO:0030015]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT core complex to near homogeneity and identified all nine curated subunits by mass spectrometry. [DOI:10.1006/jmbi.2001.5162]

Functions

poly(A)-specific ribonuclease activity GO:0004535 — CCR4-NOT supplies the major Ccr4/Caf1-dependent cytoplasmic poly(A)-specific exonuclease activity in budding yeast.
- GO:0004535 GO:0004535 is the Gene Ontology molecular-function term for poly(A)-specific ribonuclease activity.
- DOI:10.1016/s0092-8674(01)00225-2 Tucker et al. 2001 showed that Ccr4 and Caf1 are critical components of the major Saccharomyces cerevisiae cytoplasmic mRNA deadenylase.
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 — CCR4-NOT participates in deadenylation-dependent mRNA decay by shortening cytoplasmic mRNA poly(A) tails.
- GO:0000288 GO:0000288 is the Gene Ontology biological-process term for deadenylation-dependent decay of nuclear-transcribed mRNAs.
- DOI:10.1016/s0092-8674(01)00225-2 Tucker et al. 2001 showed that Ccr4 and Caf1 are required for normal mRNA deadenylation in vivo.

Mechanism graphs

CCR4-NOT supports yeast cytoplasmic mRNA deadenylation (FUNCTION)

In Saccharomyces cerevisiae, the nine-subunit 1.0 MDa CCR4-NOT core complex carries Ccr4/Caf1-dependent mRNA deadenylase activity that shortens poly(A) tails.

Subject	Predicate	Object	Evidence
Ccr4 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf1/Pop2 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not1/Cdc39 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not2/Cdc36 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not3 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not4/Mot2 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Not5 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf40 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
Caf130 CCR4-NOT core subunit	contributes to	CCR4-NOT core complex	`GO:0030015` GO:0030015 defines the Saccharomyces CCR4-NOT core complex as comprising Ccr4p, Caf1p, Caf40p, Caf130p, Not1p, Not2p, Not3p, Not4p and Not5p. `DOI:10.1006/jmbi.2001.5162` Chen et al. 2001 purified the Saccharomyces cerevisiae 1.0 MDa CCR4-NOT complex and identified CCR4, CAF1, NOT1-NOT5, CAF40 and CAF130 by mass spectrometry.
CCR4-NOT core complex	enables	poly(A)-specific ribonuclease activity	`DOI:10.1016/s0092-8674(01)00225-2` Tucker et al. 2001 purified Caf1p with a Ccr4p-dependent poly(A)-specific exonuclease activity. `GO:0004535` GO:0004535 denotes poly(A)-specific ribonuclease activity.
poly(A)-specific ribonuclease activity	supports	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	`DOI:10.1016/s0092-8674(01)00225-2` Tucker et al. 2001 established that Ccr4 and Caf1 are required for normal mRNA deadenylation in vivo. `GO:0000288` GO:0000288 defines the major deadenylation-dependent decay pathway as including poly(A)-tail shortening.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the CCR4-NOT core constituent classes. The budding-yeast CCR4-NOT core contains nuclease, NOT and Caf subunits. Exact InterPro, Pfam, NCBIfam or UniProt family CURIEs should only be curated after verifying that they denote full subunit classes rather than shared nuclease, NOT-box or WD-repeat domains.

Evidence

GO:0030015 Gene Ontology cellular-component term for the unrestricted, non-obsolete CCR4-NOT core complex.
GO:0032991 Gene Ontology cellular-component parent term for protein-containing complexes.
GO:0004535 Gene Ontology molecular-function term for poly(A)-specific ribonuclease activity.
GO:0000288 Gene Ontology biological-process term for deadenylation-dependent nuclear-transcribed mRNA decay.
PMID:11733989 PubMed identifier for Chen et al. 2001, the 1.0 MDa Saccharomyces cerevisiae CCR4-NOT core-complex purification paper.
DOI:10.1006/jmbi.2001.5162 DOI for Chen et al. 2001, 'Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex', verified through NCBI E-utilities.
PMID:11239395 PubMed identifier for Tucker et al. 2001, the budding-yeast Ccr4/Caf1 cytoplasmic mRNA deadenylase paper.
DOI:10.1016/s0092-8674(01)00225-2 DOI for Tucker et al. 2001, 'The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae', verified through NCBI E-utilities.

Curation history

2026-09-27T08:34:33Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-27T08:35:22Z codex · EXPANDED_RECORD — Added 1.0 MDa CCR4-NOT exact synonymy, protein-containing-complex parentage, nine budding-yeast CCR4-NOT core constituent rows, yeast scope, poly(A)-specific ribonuclease and mRNA decay functions, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a CCR4-NOT subunit-grounding TODO.

Source: data/structures/other/ccr4_not_core_complex.yaml