DASH complex

GO:0042729 · Other · Multiprotein complex · Proposed

A large protein complex, containing around 8-10 subunits in yeast, including Duo1p, Dam1p, Dad1p and Ask1p. The complex forms part of the outer kinetochore, associates with microtubules when the kinetochore attaches to the spindle, and plays a role in spindle attachment, chromosome segregation and spindle stability. [GO:0042729]

Synonyms: condensed nuclear chromosome kinetochore-associated DASH complex; Dam1 complex; DDD complex; Duo1p-Dam1p-Dad1p complex

Part of: outer kinetochore (GO:0000940); kinetochore (GO:0000776)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Duo1 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	DUO1	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Dam1 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	DAM1	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Dad1 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	DAD1	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Dad2 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	DAD2	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Ask1 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	ASK1	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Spc19 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	SPC19	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
Spc34 DASH subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	SPC34	UNKNOWN	Named Saccharomyces cerevisiae constituent of the DASH complex. `GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1093/emboj/21.1.181` Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Cheeseman et al. purified a Saccharomyces cerevisiae Dam1p outer-kinetochore complex that contains several DASH subunits and directly binds microtubules. [DOI:10.1083/jcb.200109063]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Reference budding-yeast system in which a Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p, and Ask1p was purified and linked to outer-kinetochore function. [DOI:10.1083/jcb.200109063]

Functions

attachment of spindle microtubules to kinetochore GO:0008608 — The budding-yeast DASH complex forms an outer-kinetochore module that binds microtubules and helps chromosomes attach to spindle microtubules.
- GO:0008608 Gene Ontology biological-process term for attachment of spindle microtubules to the kinetochore.
- GO:0042729 Gene Ontology cellular-component term for the DASH complex.
- DOI:10.1083/jcb.200109063 Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function.

Mechanism graphs

DASH links the outer kinetochore to spindle microtubules (ASSEMBLY)

In budding yeast, the DASH/Dam1 complex is an outer-kinetochore multiprotein complex that binds microtubules and contributes to kinetochore-spindle attachment.

Subject	Predicate	Object	Evidence
DASH complex	is part of	outer kinetochore	`GO:0042729` Gene Ontology cellular-component term for the DASH complex.
outer kinetochore	is part of	kinetochore	`GO:0000940` GO:0000940 defines the outer kinetochore as the kinetochore region most external to centromeric DNA.
DASH complex	contributes to	attachment of spindle microtubules to kinetochore	`GO:0042729` Gene Ontology cellular-component term for the DASH complex. `DOI:10.1083/jcb.200109063` Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function. `DOI:10.1038/nsmb896` Miranda et al. 2005 reconstituted the yeast DASH complex and showed that it can form closed rings on microtubules.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the curated DASH subunits and verify the omitted Dad3, Dad4, Hsk-family microprotein boundary. GO defines DASH as a yeast 8-10 subunit complex. This first pass curates seven subunits directly named in GO, Cheeseman 2001, and Janke 2002, and leaves tiny Dad/Hsk-family DASH subunits for a dedicated source-neutral grounding and boundary review.

Evidence

GO:0042729 Gene Ontology cellular-component term for the DASH complex.
GO:0140513 Gene Ontology cellular-component parent term for nuclear protein-containing complexes.
GO:0000940 Gene Ontology cellular-component term for the outer kinetochore, which contains the DASH complex.
GO:0000776 Gene Ontology cellular-component term for kinetochore.
GO:0008608 Gene Ontology biological-process term for spindle-microtubule attachment to the kinetochore.
DOI:10.1083/jcb.200109063 Cheeseman et al. 2001 purified a budding-yeast Dam1p complex containing Dam1p, Duo1p, Dad1p, Spc19p, Spc34p, Dad2p and Ask1p and linked the complex to outer kinetochore function.
DOI:10.1093/emboj/21.1.181 Janke et al. 2002 studied Ask1p, Dad2p, Spc19p and Spc34p as subunits of the budding-yeast Duo1p-Dam1p-Dad1p complex.
DOI:10.1038/nsmb896 Miranda et al. 2005 reconstituted the yeast DASH complex and showed that it can form closed rings on microtubules.

Curation history

2026-09-18T13:30:31Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-18T13:31:48Z codex · EXPANDED_RECORD — Added DASH synonymy, nuclear-complex parentage, outer-kinetochore parthood, a seven-subunit reviewed-label-only yeast component set, Saccharomyces scope, spindle-microtubule attachment function, GO/DOI evidence, a nonmechanistic outer-kinetochore attachment graph, and a subunit-boundary TODO.
2026-09-18T13:32:30Z codex · NORMALIZED_YAML — Rewrote the DASH record with independent evidence dictionaries so the serialized YAML does not contain anchors or aliases.
2026-09-18T13:46:24Z codex · ADDRESS_REVIEW — Narrowed the DASH Saccharomyces taxonomic-distribution and canonical-example notes so each row is directly supported by its row-level reference.
2026-09-18T13:53:23Z codex · ADDRESS_REVIEW — Weakened the DASH-to-spindle-microtubule attachment graph edge from stabilization to a contribution claim that matches the attached evidence.

Source: data/structures/other/dash_complex.yaml