DNA replication factor A complex

GO:0005662 · Other · Multiprotein complex · Proposed

A conserved heterotrimeric eukaryotic single-stranded-DNA-binding complex, also known as replication protein A, that supports DNA replication, repair and recombination. [GO:0005662]

Synonyms: replication protein A; RPA

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Essential	Role
RPA 70 kDa subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Approximately 70 kDa constituent class of the eukaryotic heterotrimeric replication protein A complex. `GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.
RPA 30 kDa subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Approximately 30 kDa constituent class of the eukaryotic heterotrimeric replication protein A complex. `GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.
RPA 14 kDa subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Approximately 14 kDa constituent class of the eukaryotic heterotrimeric replication protein A complex. `GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.

Taxonomic distribution

NCBITaxon:2759 Eukaryota — COMMON: Gene Ontology defines DNA replication factor A as a conserved eukaryotic heterotrimeric complex and constrains GO:0005662 to Eukaryota. [GO:0005662]

Functions

single-stranded DNA binding GO:0003697 — The DNA replication factor A complex binds nonspecifically to single-stranded DNA.
- GO:0005662 GO:0005662 defines DNA replication factor A as binding nonspecifically to single-stranded DNA.
- GO:0003697 GO:0003697 is the Gene Ontology molecular-function term for single-stranded DNA binding.

Mechanism graphs

RPA binds single-stranded DNA (FUNCTION)

The approximately 70, 30 and 14 kDa subunits form the eukaryotic DNA replication factor A complex that binds nonspecifically to single-stranded DNA.

Subject	Predicate	Object	Evidence
RPA 70 kDa subunit	contributes to	DNA replication factor A complex	`GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.
RPA 30 kDa subunit	contributes to	DNA replication factor A complex	`GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.
RPA 14 kDa subunit	contributes to	DNA replication factor A complex	`GO:0005662` GO:0005662 defines DNA replication factor A as a heterotrimeric eukaryotic complex composed of approximately 70, 30 and 14 kDa subunits.
DNA replication factor A complex	supports	single-stranded DNA binding	`GO:0005662` GO:0005662 defines DNA replication factor A as binding nonspecifically to single-stranded DNA. `GO:0003697` GO:0003697 is the molecular-function term for single-stranded DNA binding.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the 70, 30 and 14 kDa subunit classes of DNA replication factor A. GO:0005662 exactly identifies the heterotrimeric eukaryotic DNA replication factor A complex and its three approximate size-defined subunits, but exact source-neutral InterPro, Pfam, NCBIfam or UniProtKB family CURIEs for those subunit classes were not curated in this pass.

Evidence

GO:0005662 Gene Ontology cellular-component term for the DNA replication factor A complex.
GO:0140513 Gene Ontology cellular-component parent term for nuclear protein-containing complexes.
GO:0003697 Gene Ontology molecular-function term for single-stranded DNA binding.
PMID:9242902 PubMed identifier listed by GO:0005662 as its definition cross-reference.

Curation history

2026-09-27T16:10:02Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-27T16:10:41Z codex · EXPANDED_RECORD — Added GO RPA synonymy, nuclear protein-containing-complex parentage, three reviewed-label-only size-class constituent rows, Eukaryota scope, single-stranded DNA-binding function evidence, GO/PubMed evidence, a nonmechanistic function graph, and an RPA subunit-grounding TODO.

Source: data/structures/other/dna_replication_factor_a_complex.yaml