DnaB-DnaC-DnaT-PriA-PriC complex

GO:1990159 · Other · Nucleoprotein complex · Proposed

A protein-DNA complex consisting of the helicase loading complex DnaB-DnaC, replication restart proteins DnaT, PriA and PriC, and associated DNA. Involved in the restart of DNA replication after a stalled replication fork has been repaired. [GO:1990159]

Synonyms: DnaB-DnaC-DnaT-PriA-PriC preprimosome; phi-X174-type preprimosome

Has part: DnaB-DnaC complex (GO:1990100)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
DnaB-DnaC complex	Protein complex	part of	`GO:1990100`		UNKNOWN	Helicase-loading subcomplex that donates DnaB during PriA/PriC preprimosome assembly. `GO:1990159` GO:1990159 defines this preprimosome as containing the DnaB-DnaC helicase-loading complex. `PMID:8663105` Ng and Marians 1996 part II is cited by GO:1990159 for the DnaB-DnaC-DnaT-PriA-PriC PhiX174-type preprimosome boundary.
DnaT replication restart protein	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	dnaT	UNKNOWN	Replication-restart protein in the PriA/PriC/DnaT assembly intermediate. `GO:1990159` GO:1990159 defines DnaT as a constituent of this PriA/PriC preprimosome. `PMID:8663105` Ng and Marians 1996 part II is cited by GO:1990159 for the DnaT-containing PhiX174-type preprimosome.
PriA replication restart protein	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	priA	UNKNOWN	Primosomal protein in the PriA/PriC restart branch. `GO:1990159` GO:1990159 defines PriA as a constituent of this PriA/PriC preprimosome. `PMID:8663105` Ng and Marians 1996 part II is cited by GO:1990159 for the PriA-containing PhiX174-type preprimosome.
PriC replication restart protein	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	priC	UNKNOWN	Replication-restart protein in the PriA/PriC branch. `GO:1990159` GO:1990159 defines PriC as a constituent of this preprimosome. `PMID:8663105` Ng and Marians 1996 part II is cited by GO:1990159 for the PriC-containing PhiX174-type preprimosome.
primosome assembly site DNA	Dna	part of			UNKNOWN	Single-stranded DNA scaffold carrying the primosome assembly site. `GO:1990159` GO:1990159 defines this preprimosome as a protein-DNA complex. `PMID:8663105` Ng and Marians 1996 part II is cited by GO:1990159 for PhiX174-type primosome protein-DNA complexes.

Taxonomic distribution

NCBITaxon:562 Escherichia coli — COMMON: Ng and Marians 1996 examined PhiX174-type primosome assembly by E. coli primosomal proteins. [PMID:8663105]

Canonical examples

NCBITaxon:562 Escherichia coli — Ng and Marians 1996 examined preservation of assembled PhiX174-type primosome composition from assembly through replication in the E. coli primosome system. [PMID:8663105]

Functions

replication fork processing GO:0031297 — The DnaB-DnaC-DnaT-PriA-PriC preprimosome participates in restarting repaired stalled replication forks.
- GO:1990159 GO:1990159 defines this preprimosome by replication restart after a stalled replication fork has been repaired.
- GO:0031297 GO:0031297 is the Gene Ontology biological-process term for restoring and restarting a stalled DNA replication fork.
- PMID:8663105 Ng and Marians 1996 part II is cited by GO:1990159 for the replication-through-restart PhiX174-type preprimosome.

Mechanism graphs

DnaB-DnaC-DnaT-PriA-PriC supports fork restart (FUNCTION)

A DnaB-DnaC helicase-loading subcomplex and PriA, PriC and DnaT assemble on single-stranded primosome assembly site DNA to form a preprimosome used in replication restart.

Subject	Predicate	Object	Evidence
DnaB-DnaC complex	contributes to	DnaB-DnaC-DnaT-PriA-PriC complex	`GO:1990159` GO:1990159 includes the DnaB-DnaC helicase-loading complex.
PriA replication restart protein	contributes to	DnaB-DnaC-DnaT-PriA-PriC complex	`GO:1990159` GO:1990159 names PriA as a replication-restart constituent.
PriC replication restart protein	contributes to	DnaB-DnaC-DnaT-PriA-PriC complex	`GO:1990159` GO:1990159 names PriC as a replication-restart constituent.
DnaT replication restart protein	contributes to	DnaB-DnaC-DnaT-PriA-PriC complex	`GO:1990159` GO:1990159 names DnaT as a replication-restart constituent.
primosome assembly site DNA	scaffolds	DnaB-DnaC-DnaT-PriA-PriC complex	`GO:1990159` GO:1990159 defines this entity as a protein-DNA complex. `PMID:8663104` Ng and Marians 1996 part I analyzed PhiX174-type primosome assembly intermediate protein-DNA complexes.
DnaB-DnaC-DnaT-PriA-PriC complex	supports	replication fork processing	`GO:1990159` GO:1990159 defines this preprimosome by its involvement in replication restart after stalled replication fork repair. `GO:0031297` GO:0031297 defines replication fork processing as restoration and restart of a stalled DNA replication fork.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral DnaT, PriA, PriC and primosome assembly site DNA groundings for the DnaB-DnaC-DnaT-PriA-PriC complex. GO:1990159 defines DnaT, PriA, PriC and associated DNA as constituents of this preprimosome. Exact source-neutral InterPro, Pfam, NCBIfam, UniProt family or SO sequence-feature CURIEs for these components need narrower curation before the labels can be grounded.
INTERPRETATION (RESOLVED): Leave PriB, Rep and DnaG out of the GO:1990159 DnaB-DnaC-DnaT-PriA-PriC complex. GO:1990159 denotes the PriA/PriC/DnaT/DnaB-DnaC preprimosome. GO models the PriB-containing alternative as GO:1990158, the Rep-containing PriC alternative as GO:1990160, and DnaG-containing entities as primosomes rather than pre-primosomes.

Evidence

GO:1990159 Gene Ontology cellular-component term for the DnaB-DnaC-DnaT-PriA-PriC complex and its exact preprimosome synonymy.
GO:1990099 QuickGO reports GO:1990159 as an is-a child of GO:1990099 pre-primosome complex.
GO:1990100 Gene Ontology cellular-component term for the DnaB-DnaC helicase-loading subcomplex.
GO:1990158 Gene Ontology cellular-component term for the PriB sibling of this PriC-containing preprimosome.
GO:1990160 Gene Ontology cellular-component term for the Rep-containing PriC sibling of this PriA-containing preprimosome.
GO:0031297 Gene Ontology biological-process term for replication fork processing.
PMID:8663104 PubMed identifier for Ng and Marians 1996 part I, which analyzed ordered PhiX174-type primosome assembly intermediates.
DOI:10.1074/jbc.271.26.15642 DOI for Ng and Marians 1996, 'The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes', as reported by PubMed.
PMID:8663105 PubMed identifier for Ng and Marians 1996 part II, the Journal of Biological Chemistry paper cited by GO:1990159.
DOI:10.1074/jbc.271.26.15649 DOI for Ng and Marians 1996, 'The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication', as reported by PubMed.

Curation history

2026-10-01T08:17:20Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-10-01T08:18:23Z codex · EXPANDED_RECORD — Added GO synonymy, pre-primosome parentage, the DnaB-DnaC subcomplex and DnaT/PriA/PriC/DNA components, E. coli/PhiX174 evidence, replication-fork-processing function evidence, GO/PubMed/DOI evidence, a nonmechanistic PriC-branch restart graph, and grounding/boundary discussions.

Source: data/structures/other/dnab_dnac_dnat_pria_pric_complex.yaml