DnaB-DnaC-Rep-PriC complex

GO:1990160 · Other · Nucleoprotein complex · Proposed

A protein-DNA complex consisting of the helicase loading complex DnaB-DnaC, replication restart proteins Rep and PriC, and associated DNA. Involved in the restart of DNA replication after a stalled replication fork has been repaired. [GO:1990160]

Synonyms: DnaB-DnaC-Rep-PriC preprimosome; phi-X174-type preprimosome

Has part: DnaB-DnaC complex (GO:1990100)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
DnaB-DnaC complex	Protein complex	part of	`GO:1990100`		UNKNOWN	Helicase-loading subcomplex in the Rep/PriC preprimosome. `GO:1990160` GO:1990160 defines this preprimosome as containing the DnaB-DnaC helicase-loading complex.
Rep helicase	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	rep	UNKNOWN	Replication-restart helicase in the Rep/PriC branch. `GO:1990160` GO:1990160 defines Rep as a constituent of this preprimosome. `PMID:19941825` Guy et al. 2009 used reconstituted E. coli replisomes to study Rep helicase activity at blocked forks.
PriC replication restart protein	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	priC	UNKNOWN	Replication-restart protein in the Rep/PriC branch. `GO:1990160` GO:1990160 defines PriC as a constituent of this preprimosome.
primosome assembly site DNA	Dna	part of			UNKNOWN	Single-stranded DNA scaffold carrying the primosome assembly site. `GO:1990160` GO:1990160 defines this preprimosome as a protein-DNA complex.

Taxonomic distribution

NCBITaxon:562 Escherichia coli — COMMON: Guy et al. 2009 used reconstituted E. coli replisomes to study Rep at replication forks. [PMID:19941825]

Canonical examples

NCBITaxon:562 Escherichia coli — Guy et al. 2009 reconstituted E. coli replisomes to analyze Rep function at forks blocked by protein-DNA complexes. [PMID:19941825]

Functions

replication fork processing GO:0031297 — The DnaB-DnaC-Rep-PriC preprimosome participates in restarting repaired stalled replication forks.
- GO:1990160 GO:1990160 defines this preprimosome by replication restart after a stalled replication fork has been repaired.
- GO:0031297 GO:0031297 is the Gene Ontology biological-process term for restoring and restarting a stalled DNA replication fork.

Mechanism graphs

DnaB-DnaC-Rep-PriC supports fork restart (FUNCTION)

A DnaB-DnaC helicase-loading subcomplex and Rep and PriC assemble on single-stranded primosome assembly site DNA to form a preprimosome used in replication restart.

Subject	Predicate	Object	Evidence
DnaB-DnaC complex	contributes to	DnaB-DnaC-Rep-PriC complex	`GO:1990160` GO:1990160 includes the DnaB-DnaC helicase-loading complex.
Rep helicase	contributes to	DnaB-DnaC-Rep-PriC complex	`GO:1990160` GO:1990160 names Rep as a replication-restart constituent.
PriC replication restart protein	contributes to	DnaB-DnaC-Rep-PriC complex	`GO:1990160` GO:1990160 names PriC as a replication-restart constituent.
primosome assembly site DNA	scaffolds	DnaB-DnaC-Rep-PriC complex	`GO:1990160` GO:1990160 defines this entity as a protein-DNA complex.
DnaB-DnaC-Rep-PriC complex	supports	replication fork processing	`GO:1990160` GO:1990160 defines this preprimosome by its involvement in replication restart after stalled replication fork repair. `GO:0031297` GO:0031297 defines replication fork processing as restoration and restart of a stalled DNA replication fork.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral Rep, PriC and primosome assembly site DNA groundings for the DnaB-DnaC-Rep-PriC complex. GO:1990160 defines Rep, PriC and associated DNA as constituents of this preprimosome. Exact source-neutral InterPro, Pfam, NCBIfam, UniProt family or SO sequence-feature CURIEs for these components need narrower curation before the labels can be grounded.
INTERPRETATION (RESOLVED): Leave PriA, PriB, DnaT and DnaG out of the GO:1990160 DnaB-DnaC-Rep-PriC complex. GO:1990160 denotes the Rep/PriC/DnaB-DnaC preprimosome. GO models PriA/PriB/DnaT and PriA/PriC/DnaT alternatives as separate sibling terms and models DnaG-containing entities as primosomes rather than pre-primosomes.

Evidence

GO:1990160 Gene Ontology cellular-component term for the DnaB-DnaC-Rep-PriC complex and its exact preprimosome synonymy.
GO:1990099 QuickGO reports GO:1990160 as an is-a child of GO:1990099 pre-primosome complex.
GO:1990100 Gene Ontology cellular-component term for the DnaB-DnaC helicase-loading subcomplex.
GO:1990158 Gene Ontology cellular-component term for the PriA/PriB/DnaT preprimosome sibling.
GO:1990159 Gene Ontology cellular-component term for the PriA/PriC/DnaT preprimosome sibling.
GO:0031297 Gene Ontology biological-process term for replication fork processing.
PMID:19941825 PubMed identifier for Guy et al. 2009, the Molecular Cell paper cited by GO:1990160.
DOI:10.1016/j.molcel.2009.11.009 DOI for Guy et al. 2009, 'Rep provides a second motor at the replisome to promote duplication of protein-bound DNA', as reported by PubMed.
PMID:8663105 PubMed identifier for Ng and Marians 1996 part II, the second paper cited by GO:1990160.
DOI:10.1074/jbc.271.26.15649 DOI for Ng and Marians 1996, 'The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication', as reported by PubMed.

Curation history

2026-10-01T08:46:25Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-10-01T08:47:15Z codex · EXPANDED_RECORD — Added GO synonymy, pre-primosome parentage, the DnaB-DnaC subcomplex and Rep/PriC/DNA components, Escherichia coli Rep evidence, replication-fork-processing function evidence, GO/PubMed/DOI evidence, a nonmechanistic Rep/PriC-branch restart graph, and grounding/boundary discussions.

Source: data/structures/other/dnab_dnac_rep_pric_complex.yaml