Ino80 complex

GO:0031011 · Other · Multiprotein complex · Proposed

A multisubunit protein complex that contains the Ino80p ATPase; exhibits chromatin remodeling activity. [GO:0031011]

Synonyms: INO80 chromatin remodeling complex; INO80-C; Ino80.com

Part of: nuclear chromosome (GO:0000228)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Ino80 INO80-C ATPase subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	INO80	UNKNOWN	Snf2-family ATPase constituent and scaffold of the budding-yeast INO80 chromatin remodeling complex. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Rvb1 INO80-C head subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RVB1	UNKNOWN	RuvB-like AAA+ ATPase constituent of the INO80-C Rvb1/Rvb2 head module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Rvb2 INO80-C head subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RVB2	UNKNOWN	RuvB-like AAA+ ATPase constituent of the INO80-C Rvb1/Rvb2 head module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies5 INO80-C subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES5	UNKNOWN	Protein constituent of the budding-yeast INO80 chromatin remodeling complex. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies3 INO80-C Nhp10 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES3	UNKNOWN	Protein constituent associated with the INO80-C Nhp10 nucleosome-binding module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Nhp10 INO80-C Nhp10 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	NHP10	UNKNOWN	Protein constituent of the high-affinity nucleosome-binding Nhp10 module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies6 INO80-C Arp5 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES6	UNKNOWN	Protein constituent of the Arp5-Ies6 module required for INO80-C remodeling. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies1 INO80-C subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES1	UNKNOWN	Protein constituent of the budding-yeast INO80 chromatin remodeling complex. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Arp8 INO80-C Arp8 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	ARP8	UNKNOWN	Actin-related-protein constituent of the INO80-C Arp8/Arp4/Act1 module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Arp4 INO80-C Arp8 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	ARP4	UNKNOWN	Actin-related-protein constituent of the INO80-C Arp8/Arp4/Act1 module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Act1 INO80-C actin constituent	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	ACT1	UNKNOWN	Actin constituent of the INO80-C Arp8/Arp4/Act1 module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Taf14 INO80-C subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	TAF14	UNKNOWN	Protein constituent of the budding-yeast INO80 chromatin remodeling complex. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies2 INO80-C neck subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES2	UNKNOWN	Protein constituent that maps near the INO80-C neck with the Arp5 module. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Ies4 INO80-C subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	IES4	UNKNOWN	Protein constituent of the budding-yeast INO80 chromatin remodeling complex. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.
Arp5 INO80-C Arp5 module subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	ARP5	UNKNOWN	Actin-related-protein constituent of the Arp5-Ies6 module required for INO80-C remodeling. `DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 reports this protein among the Saccharomyces cerevisiae INO80-C subunits detected by mass spectrometry in three independent TAP-tagged purifications.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the source organism for the original INO80 purification and for the 15-subunit INO80-C mass spectrometry preparations. [DOI:10.1038/ncomms8108]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Shen et al. 2000 purified the budding-yeast Ino80 ATPase complex and showed that the complex remodels chromatin in vitro. [DOI:10.1038/35020123]

Functions

chromatin remodeling GO:0006338 — The Ino80 complex is an ATP-dependent INO80-type chromatin-remodeling complex.
- GO:0006338 GO:0006338 is the Gene Ontology biological-process term for chromatin remodeling.
- DOI:10.1038/35020123 Shen et al. 2000 purified the budding-yeast Ino80 ATPase complex and assayed its chromatin-remodeling activity.

Mechanism graphs

INO80-C carries yeast chromatin remodeling (FUNCTION)

In Saccharomyces cerevisiae, Ino80, Rvb1, Rvb2, Ies5, Ies3, Nhp10, Ies6, Ies1, Arp8, Arp4, Act1, Taf14, Ies2, Ies4 and Arp5 form the INO80-C chromatin-remodeling complex.

Subject	Predicate	Object	Evidence
Ino80 INO80-C ATPase subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Rvb1 INO80-C head subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Rvb2 INO80-C head subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies5 INO80-C subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies3 INO80-C Nhp10 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Nhp10 INO80-C Nhp10 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies6 INO80-C Arp5 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies1 INO80-C subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Arp8 INO80-C Arp8 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Arp4 INO80-C Arp8 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Act1 INO80-C actin constituent	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Taf14 INO80-C subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies2 INO80-C neck subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ies4 INO80-C subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Arp5 INO80-C Arp5 module subunit	contributes to	Ino80 complex	`DOI:10.1038/ncomms8108` Watanabe et al. 2015 Supplementary Table 2 lists this protein among the purified yeast INO80-C subunits.
Ino80 complex	enables	chromatin remodeling	`DOI:10.1038/35020123` Shen et al. 2000 purified the Ino80 complex from Saccharomyces cerevisiae and assayed its chromatin-remodeling activity. `GO:0006338` GO:0006338 lists GO:0031011 as a cellular component capable of chromatin remodeling.

Discussions

CURATION_TODO (OPEN): Curate exact source-neutral InterPro, Pfam, NCBIfam or UniProt family groundings for the 15 INO80-C subunit classes when terms that denote the component classes, rather than broader domains, can be verified. Several INO80-C constituents are shared with SWR-C or other transcription and chromatin complexes. Exact groundings should distinguish INO80-C component classes from broader ATPase, actin-related protein, actin, or YEATS domain families.

Evidence

GO:0031011 Gene Ontology cellular-component term for the Ino80 complex and its exact INO80 chromatin remodeling complex synonym.
GO:0097346 Gene Ontology cellular-component parent term for INO80-type complexes.
GO:0000228 Gene Ontology cellular-component term for the nuclear chromosome.
GO:0006338 Gene Ontology biological-process term for chromatin remodeling.
PMID:10952318 PubMed identifier for Shen et al. 2000, the Nature paper that first purified the budding-yeast INO80 complex.
DOI:10.1038/35020123 DOI for Shen et al. 2000, 'A chromatin remodelling complex involved in transcription and DNA processing', verified through NCBI E-utilities.
PMID:12887900 PubMed identifier for Shen et al. 2003, the Molecular Cell paper that defined Arp4, Arp5, Arp8 and Act1 as stoichiometric INO80 constituents.
DOI:10.1016/s1097-2765(03)00264-8 DOI for Shen et al. 2003, 'Involvement of actin-related proteins in ATP-dependent chromatin remodeling', verified through NCBI E-utilities.
PMID:15525518 PubMed identifier for Jonsson et al. 2004, the Molecular Cell paper on Rvb1p/Rvb2p recruitment of Arp5p into Ino80.com.
DOI:10.1016/j.molcel.2004.09.033 DOI for Jonsson et al. 2004, 'Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex', verified through NCBI E-utilities.
PMID:19355820 PubMed identifier for Clapier and Cairns 2009, the Annual Review of Biochemistry chromatin-remodeling review cited by GO:0031011 and GO:0097346.
DOI:10.1146/annurev.biochem.77.062706.153223 DOI for Clapier and Cairns 2009, 'The biology of chromatin remodeling complexes', verified through NCBI E-utilities.
PMID:24034245 PubMed identifier for Tosi et al. 2013, the Cell paper on yeast INO80 complex structure and subunit topology.
DOI:10.1016/j.cell.2013.08.016 DOI for Tosi et al. 2013, 'Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex', verified through NCBI E-utilities.
PMID:25964121 PubMed identifier for Watanabe et al. 2015, the Nature Communications structural analysis with INO80-C mass spectrometry stoichiometry.
DOI:10.1038/ncomms8108 DOI for Watanabe et al. 2015, 'Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C', verified through NCBI E-utilities.

Curation history

2026-09-27T01:24:50Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-27T01:27:47Z codex · EXPANDED_RECORD — Added INO80 exact synonymy, INO80-type parentage, nuclear-chromosome parthood, 15 budding-yeast constituent rows, yeast scope, chromatin-remodeling function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a subunit-grounding TODO.

Source: data/structures/other/ino80_complex.yaml