ModE complex

GO:1990198 · Other · Multiprotein complex · Proposed

A dimeric protein complex containing two ModE subunits. Binds directly to DNA to regulate transcription, and is involved in (positively and negatively) regulating various aspects of molybdenum metabolism. [GO:1990198]

Synonyms: ModE dimer

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Molybdate-dependent transcriptional regulator ModE	Protein	part of	`InterPro:IPR016462`	modE, modR	2 copies per E. coli ModE homodimer in PDB:1O7L biological assembly 1	UNKNOWN	Homodimeric transcription-regulator subunit with an N-terminal DNA-binding domain and C-terminal oxyanion-binding domains that bind molybdate. `GO:1990198` GO:1990198 defines the ModE complex as a dimeric protein complex containing two ModE subunits. `InterPro:IPR016462` InterPro IPR016462 is the full-length molybdate-dependent transcriptional regulator ModE family entry. `PDB:1O7L` RCSB describes each PDB:1O7L biological assembly as a homomeric E. coli ModE dimer.

Organism-specific protein examples

Named proteins in which each component's role was established, with the organism the evidence comes from. The component above is the taxon-agnostic family; these are the specific entries behind it.

Molybdate-dependent transcriptional regulator ModE

Accession	Protein	Gene	Organism	Entry
`UniProtKB:P0A9G8`	DNA-binding transcriptional dual regulator ModE	modE	Escherichia coli K-12 (`NCBITaxon:83333`)	REVIEWED
Role and evidence (2 references) Reviewed E. coli K-12 ModE accession that UniProt maps to GO:1990198 and RCSB maps to the ModE homodimer in PDB:1O7L. `UniProtKB:P0A9G8` UniProtKB P0A9G8 is the reviewed E. coli K-12 entry for DNA-binding transcriptional dual regulator ModE. `PDB:1O7L` RCSB reports PDB:1O7L as molybdate-activated E. coli ModE with homomeric dimer biological assemblies.

Canonical examples

NCBITaxon:562 Escherichia coli — E. coli source of the molybdate-activated ModE homodimer deposited as PDB:1O7L. [PDB:1O7L]

Functions

molybdenum ion binding GO:0030151 — The ModE homodimer binds molybdate through its C-terminal oxyanion-binding domains.
- GO:1990198 GO:1990198 defines the complex by its involvement in molybdenum metabolism.
- GO:0030151 GO:0030151 is the Gene Ontology molecular-function term for molybdenum ion binding.
- PDB:1O7L RCSB reports PDB:1O7L as the molybdate-activated form of E. coli ModE.
cis-regulatory region sequence-specific DNA binding GO:0000987 — The ModE homodimer uses its N-terminal DNA-binding domains to bind regulatory DNA sites.
- GO:1990198 GO:1990198 defines the ModE complex as binding DNA directly to regulate transcription.
- GO:0000987 GO:0000987 is the Gene Ontology molecular-function term for cis-regulatory region sequence-specific DNA binding.

Physical properties

SUBUNIT_COUNT: 2 (E. coli ModE homodimer in PDB:1O7L biological assembly 1) [PDB:1O7L]

Evidence

GO:1990198 Gene Ontology cellular-component term for the dimeric ModE complex and source of the exact ModE dimer synonym.
GO:0005667 QuickGO reports GO:1990198 as an is-a child of GO:0005667 transcription regulator complex.
GO:0030151 Gene Ontology molecular-function term for molybdenum ion binding.
GO:0000987 Gene Ontology molecular-function term for cis-regulatory region sequence-specific DNA binding.
InterPro:IPR016462 InterPro family entry for molybdate-dependent transcriptional regulators of the ModE type.
UniProtKB:P0A9G8 UniProtKB reviewed E. coli K-12 ModE entry.
PDB:1O7L RCSB Protein Data Bank entry for the molybdate-activated homodimeric E. coli ModE structure.
PMID:12581638 PubMed identifier for Schuttelkopf, Boxer and Hunter 2003, the Journal of Molecular Biology ModE structural paper cited by PDB:1O7L.
DOI:10.1016/S0022-2836(02)01358-X DOI for Schuttelkopf, Boxer and Hunter 2003, 'Crystal Structure of Activated ModE Reveals Conformational Changes Involving Both Oxyanion and DNA-binding Domains', as reported by Crossref.

Curation history

2026-10-01T22:05:05Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-10-01T22:06:35Z codex · EXPANDED_RECORD — Added GO exact synonymy and transcription-regulator parentage, an InterPro-grounded ModE constituent with a reviewed E. coli K-12 UniProt example, an E. coli PDB-backed canonical example, molybdenum-binding and DNA-binding functions, dimer subunit count, and DOI/PMID/PDB evidence.

Source: data/structures/other/mode_complex.yaml