NatC complex

GO:0031417 · Other · Multiprotein complex · Proposed

A conserved complex that catalyzes the transfer of an acetyl group to the N-terminal residue of a protein acceptor molecule that has a Met-Ile, Met-Leu, Met-Trp, or Met-Phe N-terminus. In Saccharomyces the complex includes Mak3p, Mak10p, and Mak31p. [GO:0031417]

Synonyms: N-terminal acetyltransferase C complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Mak3 NatC catalytic subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	MAK3	UNKNOWN	Catalytic constituent of the budding-yeast NatC complex that works with Mak10 and Mak31 to form active NatC. `DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.
Mak10 NatC auxiliary subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	MAK10	UNKNOWN	Auxiliary constituent of the budding-yeast NatC complex that associates with Mak3 and Mak31 to form active NatC. `DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.
Mak31 NatC auxiliary subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	MAK31	UNKNOWN	Auxiliary constituent of the budding-yeast NatC complex that associates with Mak3 and Mak10 to form active NatC. `DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Polevoda and Sherman 2001 characterized Mak3p, Mak10p and Mak31p as associated NatC subunits in Saccharomyces cerevisiae. [DOI:10.1074/jbc.M011440200]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Budding yeast is the source organism in which Mak3p, Mak10p and Mak31p were shown to associate into active NatC. [DOI:10.1074/jbc.M011440200]

Functions

protein-N-terminal amino-acid acetyltransferase activity GO:0004596 — Transfer of an acetyl group from acetyl-CoA to the N-terminal alpha-amino group of NatC substrate proteins.
- GO:0004596 GO:0004596 is the Gene Ontology molecular-function term for protein-N-terminal amino-acid acetyltransferase activity.
- GO:0031417 GO:0031417 defines the NatC complex by transfer of acetyl groups to N-terminal Met-Ile, Met-Leu, Met-Trp or Met-Phe protein residues.
- DOI:10.1074/jbc.M011440200 Polevoda and Sherman 2001 show that the Mak3p, Mak10p and Mak31p subunits associate to form active yeast NatC and that deleting any one subunit disrupts in vivo acetylation of a NatC substrate.

Mechanism graphs

NatC carries yeast N-terminal acetyltransferase activity (FUNCTION)

In Saccharomyces cerevisiae, Mak3, Mak10 and Mak31 form a NatC complex that supports protein-N-terminal acetyltransferase activity.

Subject	Predicate	Object	Evidence
Mak3 NatC catalytic subunit	contributes to	NatC complex	`DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.
Mak10 NatC auxiliary subunit	contributes to	NatC complex	`DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.
Mak31 NatC auxiliary subunit	contributes to	NatC complex	`DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 report that Saccharomyces cerevisiae NatC contains Mak3p, Mak10p and Mak31p, and that all three subunits associate to form the active complex. `DOI:10.1016/s0006-291x(03)01316-0` Polevoda and Sherman 2003 review the eukaryotic N-terminal acetyltransferase complexes and describe yeast NatC as Mak3p with the Mak10 and Mak31p auxiliary subunits.
NatC complex	supports	protein-N-terminal amino-acid acetyltransferase activity	`GO:0004596` GO:0004596 is the molecular-function term for protein-N-terminal amino-acid acetyltransferase activity. `DOI:10.1074/jbc.M011440200` Polevoda and Sherman 2001 show that Mak3p, Mak10p and Mak31p are required to form active yeast NatC.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the curated budding-yeast NatC constituents.

Evidence

GO:0031417 Gene Ontology cellular-component term for NatC complex and its exact N-terminal acetyltransferase C complex synonym.
GO:0031414 Gene Ontology cellular-component parent term for N-terminal protein acetyltransferase complexes.
GO:0004596 Gene Ontology molecular-function term for protein-N-terminal amino-acid acetyltransferase activity.
PMID:11274203 PubMed identifier for Polevoda and Sherman 2001, the budding-yeast NatC composition paper.
DOI:10.1074/jbc.M011440200 DOI for Polevoda and Sherman 2001, 'NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p', verified through NCBI E-utilities.
PMID:12890471 PubMed identifier for Polevoda and Sherman 2003, the eukaryotic N-terminal acetyltransferase subunit review cited by GO:0031417.
DOI:10.1016/s0006-291x(03)01316-0 DOI for Polevoda and Sherman 2003, 'Composition and function of the eukaryotic N-terminal acetyltransferase subunits', verified through NCBI E-utilities.

Curation history

2026-09-26T09:50:13Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T09:51:13Z codex · EXPANDED_RECORD — Added NatC exact synonymy, N-terminal protein acetyltransferase parentage, three budding-yeast constituent rows, yeast scope, protein-N-terminal acetyltransferase function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a subunit-grounding TODO.

Source: data/structures/other/natc_complex.yaml