NuA4 histone acetyltransferase complex

GO:0035267 · Other · Multiprotein complex · Proposed

A complex having histone acetylase activity on chromatin, as well as ATPase, DNA helicase and structural DNA binding activities. The complex is thought to be involved in double-strand DNA break repair. Subunits of the human complex include HTATIP/TIP60, TRRAP, RUVBL1, BUVBL2, beta-actin and BAF53/ACTL6A. In yeast, the complex has 13 subunits, including the catalytic subunit Esa1 (homologous to human Tip60). [GO:0035267]

Synonyms: TIP60 histone acetylase complex; TIP60 histone acetyltransferase complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Essential	Role
Tra1 NuA4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	PIKK-family pseudokinase and scaffolding constituent of the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Eaf1 NuA4 scaffold subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	NuA4-specific scaffold constituent that coordinates Tra1, TINTIN, piccolo-NuA4 and Arp4/Act1/Swc4/Yaf9 modules. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Eaf3 NuA4 TINTIN subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Protein constituent of the Eaf5/Eaf7/Eaf3 TINTIN module in the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Eaf5 NuA4 TINTIN subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Protein constituent of the Eaf5/Eaf7/Eaf3 TINTIN module in the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Eaf7 NuA4 TINTIN subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Protein constituent of the Eaf5/Eaf7/Eaf3 TINTIN module in the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Esa1 NuA4 catalytic subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Catalytic MYST-family histone acetyltransferase constituent of the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Epl1 NuA4 piccolo subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Piccolo-NuA4 constituent that bridges Esa1 and Yng2 in the catalytic module. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Yng2 NuA4 piccolo subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	ING-family piccolo-NuA4 constituent of the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Eaf6 NuA4 piccolo subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Piccolo-NuA4 constituent of the budding-yeast NuA4 complex. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Act1 NuA4 actin constituent	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Actin constituent of the budding-yeast NuA4 Arp4/Act1/Swc4/Yaf9 module. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Arp4 NuA4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Actin-related protein constituent of the budding-yeast NuA4 Arp4/Act1/Swc4/Yaf9 module. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Swc4 NuA4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Eaf2/Swc4 constituent of the budding-yeast NuA4 Arp4/Act1/Swc4/Yaf9 module. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.
Yaf9 NuA4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	UNKNOWN	Protein constituent of the budding-yeast NuA4 Arp4/Act1/Swc4/Yaf9 module. `DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe yeast NuA4 as a 13-subunit complex containing Tra1, Eaf1, Eaf3, Eaf5, Eaf7, Esa1, Epl1, Yng2, Eaf6, actin, Arp4, Swc4 and Yaf9.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which Wang et al. 2018 resolved the modular architecture of the 13-subunit NuA4/TIP60 histone acetyltransferase complex. [DOI:10.1038/s41467-018-03504-5]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Wang et al. 2018 purified endogenous budding-yeast NuA4 and resolved Tra1/Eaf1-centered TEEAA and TEEAA-piccolo assemblies by cryo-EM. [DOI:10.1038/s41467-018-03504-5]

Functions

histone acetyltransferase activity GO:0004402 — The NuA4 complex acetylates histone H4 and H2A N-terminal tails through its Esa1 catalytic subunit.
- GO:0035267 GO:0035267 defines NuA4/TIP60 as a complex having histone acetylase activity on chromatin.
- GO:0004402 GO:0004402 is the Gene Ontology molecular-function term for histone acetyltransferase activity.
- DOI:10.1128/MCB.24.5.1884-1896.2004 Doyon et al. 2004 describe the yeast NuA4 multisubunit complex as responsible for acetylating histone H4 and H2A N-terminal tails.

Mechanism graphs

NuA4 carries yeast histone acetyltransferase activity (FUNCTION)

In Saccharomyces cerevisiae, thirteen constituents assemble into a NuA4/TIP60 complex organized around Tra1 and Eaf1 and carrying Esa1-dependent histone acetylation.

Subject	Predicate	Object	Evidence
Tra1 NuA4 subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Eaf1 NuA4 scaffold subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Eaf3 NuA4 TINTIN subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Eaf5 NuA4 TINTIN subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Eaf7 NuA4 TINTIN subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Esa1 NuA4 catalytic subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Epl1 NuA4 piccolo subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Yng2 NuA4 piccolo subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Eaf6 NuA4 piccolo subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Act1 NuA4 actin constituent	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Arp4 NuA4 subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Swc4 NuA4 subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
Yaf9 NuA4 subunit	contributes to	NuA4 histone acetyltransferase complex	`DOI:10.1038/s41467-018-03504-5` Wang et al. 2018 describe the 13-subunit modular architecture of budding-yeast NuA4.
NuA4 histone acetyltransferase complex	supports	histone acetyltransferase activity	`GO:0035267` GO:0035267 defines NuA4/TIP60 as a complex having histone acetylase activity on chromatin. `GO:0004402` GO:0004402 is the molecular-function term for histone acetyltransferase activity. `DOI:10.1128/MCB.24.5.1884-1896.2004` Doyon et al. 2004 describe yeast NuA4 as a histone H4/H2A acetyltransferase complex.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the NuA4 histone acetyltransferase complex constituents. Wang et al. 2018 define the 13-subunit budding-yeast NuA4/TIP60 architecture. Exact cross-eukaryotic InterPro, Pfam, NCBIfam or UniProt family boundaries for each NuA4 subunit class need narrower curation before the component labels can be grounded.

Evidence

GO:0035267 Gene Ontology cellular-component term for the NuA4/TIP60 histone acetyltransferase complex and its exact TIP60 synonyms.
GO:0043189 Gene Ontology cellular-component parent term for H4/H2A histone acetyltransferase complexes.
GO:0004402 Gene Ontology molecular-function term for histone acetyltransferase activity.
PMID:14966270 PubMed identifier for Doyon et al. 2004, the conserved NuA4/TIP60 paper cited by GO:0035267.
DOI:10.1128/MCB.24.5.1884-1896.2004 DOI for Doyon et al. 2004, 'Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans', verified through NCBI E-utilities.
PMID:29559617 PubMed identifier for Wang et al. 2018, the Saccharomyces cerevisiae NuA4/TIP60 architecture paper.
DOI:10.1038/s41467-018-03504-5 DOI for Wang et al. 2018, 'Architecture of the Saccharomyces cerevisiae NuA4/TIP60 complex', verified through NCBI E-utilities.

Curation history

2026-09-26T05:45:40Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T05:46:55Z codex · EXPANDED_RECORD — Added NuA4/TIP60 exact synonymy and histone-acetyltransferase-complex parentage, thirteen budding-yeast NuA4 constituent rows, yeast scope, histone acetyltransferase activity, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a NuA4 subunit-grounding TODO.
2026-09-26T05:48:31Z codex · NARROWED_PARENT — Replaced broad histone acetyltransferase complex parentage with the direct H4/H2A histone acetyltransferase complex parent.

Source: data/structures/other/nua4_histone_acetyltransferase_complex.yaml