nucleotide-excision repair factor 1 complex

GO:0000110 · Other · Multiprotein complex · Proposed

One of several protein complexes involved in nucleotide-excision repair; possesses DNA damage recognition and endodeoxynuclease activities. In S. cerevisiae, it is composed of Rad1p, Rad10p, and Rad14p; in human the subunits are ERCC4/XPF, ERCC1 and XPA, respectively. [GO:0000110]

Synonyms: NEF1 complex; XPA-ERCC1-ERCC4 complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Rad1 NEF1 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD1	UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 1 / NEF1 complex. `GO:0000110` GO:0000110 defines the NEF1 complex as containing Rad1p.
Rad10 NEF1 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD10	UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 1 / NEF1 complex. `GO:0000110` GO:0000110 defines the NEF1 complex as containing Rad10p.
Rad14 NEF1 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD14	UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 1 / NEF1 complex. `GO:0000110` GO:0000110 defines the NEF1 complex as containing Rad14p.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which GO:0000110 defines NEF1 as a nucleotide-excision repair factor complex with Rad1p, Rad10p and Rad14p constituents. [GO:0000110]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Prakash and Prakash 2000 described budding-yeast Rad14 and Rad1-Rad10 as one nucleotide-excision repair subassembly called NEF1. [DOI:10.1016/s0027-5107(00)00037-3]

Functions

nucleotide-excision repair GO:0006289 — The NEF1 complex participates in nucleotide-excision repair as a DNA-damage-recognition and endodeoxynuclease-containing protein complex.
- GO:0000110 GO:0000110 defines NEF1 as a nucleotide-excision repair factor with DNA damage recognition and endodeoxynuclease activities.
- GO:0006289 GO:0006289 is the Gene Ontology biological-process term for nucleotide-excision repair.
- DOI:10.1016/s0027-5107(00)00037-3 Prakash and Prakash 2000 described Rad14 and the Rad1-Rad10 complex as the budding-yeast NEF1 multiprotein subassembly.

Mechanism graphs

NEF1 is a yeast nucleotide-excision repair factor (FUNCTION)

In Saccharomyces cerevisiae, Rad1p, Rad10p and Rad14p form the NEF1 subassembly of the nucleotide-excision repair protein system.

Subject	Predicate	Object	Evidence
Rad1 NEF1 subunit	contributes to	NEF1 complex	`GO:0000110` GO:0000110 defines Rad1p as a component of the NEF1 complex.
Rad10 NEF1 subunit	contributes to	NEF1 complex	`GO:0000110` GO:0000110 defines Rad10p as a component of the NEF1 complex.
Rad14 NEF1 subunit	contributes to	NEF1 complex	`GO:0000110` GO:0000110 defines Rad14p as a component of the NEF1 complex.
NEF1 complex	supports	nucleotide-excision repair	`GO:0000110` GO:0000110 defines NEF1 as one of several complexes involved in nucleotide-excision repair. `GO:0006289` GO:0006289 is the process term for nucleotide-excision repair. `DOI:10.1016/s0027-5107(00)00037-3` Prakash and Prakash 2000 described NEF1 as an in-vivo multiprotein subassembly of the yeast NER proteins.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the Rad1, Rad10 and Rad14 constituents of the NEF1 complex. GO:0000110 defines budding-yeast Rad1p, Rad10p and Rad14p constituents and also maps the corresponding human subunits as ERCC4/XPF, ERCC1 and XPA. Exact cross-eukaryotic InterPro, Pfam, NCBIfam or UniProt family boundaries for these NER factor classes need narrower curation before these component labels can be grounded.

Evidence

GO:0000110 Gene Ontology cellular-component term for the NEF1 complex and its exact NEF1 and XPA-ERCC1-ERCC4 synonyms.
GO:0000109 Gene Ontology cellular-component parent term for nucleotide-excision repair complexes.
GO:0006289 Gene Ontology biological-process term for nucleotide-excision repair.
PMID:10915862 PubMed identifier for Prakash and Prakash 2000, the Mutation Research yeast nucleotide-excision repair review cited by GO:0000110.
DOI:10.1016/s0027-5107(00)00037-3 DOI for Prakash and Prakash 2000, 'Nucleotide excision repair in yeast', verified through NCBI E-utilities.

Curation history

2026-09-26T01:51:21Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T01:52:24Z codex · EXPANDED_RECORD — Added NEF1 and XPA-ERCC1-ERCC4 exact synonymy, nucleotide-excision repair complex parentage, three reviewed-label-only Rad constituent rows, budding-yeast scope, a nucleotide-excision-repair function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a NEF1 subunit-grounding TODO.

Source: data/structures/other/nucleotide_excision_repair_factor_1_complex.yaml