nucleotide-excision repair factor 2 complex

GO:0000111 · Other · Multiprotein complex · Proposed

One of several protein complexes involved in nucleotide-excision repair; possesses damaged DNA binding activity. In S. cerevisiae, it is composed of Rad4p and Rad23p. [GO:0000111]

Synonyms: NEF2 complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Rad4 NEF2 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD4		UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 2 / NEF2 complex. `GO:0000111` GO:0000111 defines the NEF2 complex as containing Rad4p.
Rad23 NEF2 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD23		UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 2 / NEF2 complex. `GO:0000111` GO:0000111 defines the NEF2 complex as containing Rad23p.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which GO:0000111 defines NEF2 as a Rad4p/Rad23p nucleotide-excision repair factor complex. [GO:0000111]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Prakash and Prakash 2000 described the budding-yeast Rad4-Rad23 complex as the nucleotide-excision repair subassembly called NEF2. [DOI:10.1016/s0027-5107(00)00037-3]

Functions

nucleotide-excision repair GO:0006289 — The NEF2 complex participates in nucleotide-excision repair as a damaged-DNA-binding protein complex.
- GO:0000111 GO:0000111 defines NEF2 as a nucleotide-excision repair factor with damaged DNA binding activity.
- GO:0006289 GO:0006289 is the Gene Ontology biological-process term for nucleotide-excision repair.
- DOI:10.1016/s0027-5107(00)00037-3 Prakash and Prakash 2000 described the Rad4-Rad23 complex as the budding-yeast NEF2 multiprotein subassembly.

Mechanism graphs

NEF2 is a yeast nucleotide-excision repair factor (FUNCTION)

In Saccharomyces cerevisiae, Rad4p and Rad23p form the NEF2 subassembly of the nucleotide-excision repair protein system.

Subject	Predicate	Object	Evidence
Rad4 NEF2 subunit	contributes to	NEF2 complex	`GO:0000111` GO:0000111 defines Rad4p as a component of the NEF2 complex.
Rad23 NEF2 subunit	contributes to	NEF2 complex	`GO:0000111` GO:0000111 defines Rad23p as a component of the NEF2 complex.
NEF2 complex	supports	nucleotide-excision repair	`GO:0000111` GO:0000111 defines NEF2 as one of several complexes involved in nucleotide-excision repair. `GO:0006289` GO:0006289 is the process term for nucleotide-excision repair. `DOI:10.1016/s0027-5107(00)00037-3` Prakash and Prakash 2000 described NEF2 as an in-vivo multiprotein subassembly of the yeast NER proteins.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the Rad4 and Rad23 constituents of the NEF2 complex. GO:0000111 defines budding-yeast Rad4p and Rad23p as the NEF2 constituents. Exact cross-eukaryotic InterPro, Pfam, NCBIfam or UniProt family boundaries for these NER factor classes need narrower curation before these component labels can be grounded.

Evidence

GO:0000111 Gene Ontology cellular-component term for the NEF2 complex and its exact NEF2 synonym.
GO:0000109 Gene Ontology cellular-component parent term for nucleotide-excision repair complexes.
GO:0006289 Gene Ontology biological-process term for nucleotide-excision repair.
PMID:10915862 PubMed identifier for Prakash and Prakash 2000, the Mutation Research yeast nucleotide-excision repair review cited by GO:0000111.
DOI:10.1016/s0027-5107(00)00037-3 DOI for Prakash and Prakash 2000, 'Nucleotide excision repair in yeast', verified through NCBI E-utilities.

Curation history

2026-09-26T02:15:16Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T02:15:16Z codex · EXPANDED_RECORD — Added NEF2 exact synonymy, nucleotide-excision repair complex parentage, two reviewed-label-only Rad constituent rows, budding-yeast scope, a nucleotide-excision-repair function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a NEF2 subunit-grounding TODO.

Source: data/structures/other/nucleotide_excision_repair_factor_2_complex.yaml