nucleotide-excision repair factor 3 complex

GO:0000112 · Other · Multiprotein complex · Proposed

One of several protein complexes involved in nucleotide-excision repair; possesses endodeoxynuclease and DNA helicase activities. In S. cerevisiae, it is composed of Rad2p and the core TFIIH-Ssl2p complex (core TFIIH is composed of Rad3p, Tfb1p, Tfb2p, Ssl1p, Tfb4p and Tfb5p. Note that Ssl2p is also called Rad25p). [GO:0000112]

Synonyms: NEF3 complex

Has part: core TFIIH complex portion of NEF3 complex (GO:0000440)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Rad2 NEF3 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD2		UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 3 / NEF3 complex. `GO:0000112` GO:0000112 defines the NEF3 complex as containing Rad2p.
core TFIIH complex portion of NEF3 complex	Protein complex	part of	`GO:0000440`			UNKNOWN	Core TFIIH complex portion that is part of the budding-yeast NEF3 complex. `GO:0000112` GO:0000112 defines the NEF3 complex as containing the core TFIIH-Ssl2p complex. `GO:0000440` GO:0000440 denotes the core TFIIH complex portion when it is part of the NEF3 complex.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which GO:0000112 defines NEF3 as a Rad2p/core-TFIIH nucleotide-excision repair factor complex. [GO:0000112]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Prakash and Prakash 2000 described budding-yeast Rad2 and TFIIH as the nucleotide-excision repair subassembly called NEF3. [DOI:10.1016/s0027-5107(00)00037-3]

Functions

nucleotide-excision repair GO:0006289 — The NEF3 complex participates in nucleotide-excision repair as an endodeoxynuclease- and DNA-helicase-containing protein complex.
- GO:0000112 GO:0000112 defines NEF3 as a nucleotide-excision repair factor with endodeoxynuclease and DNA helicase activities.
- GO:0006289 GO:0006289 is the Gene Ontology biological-process term for nucleotide-excision repair.
- DOI:10.1016/s0027-5107(00)00037-3 Prakash and Prakash 2000 described Rad2 and TFIIH as the budding-yeast NEF3 multiprotein subassembly.

Mechanism graphs

NEF3 is a yeast nucleotide-excision repair factor (FUNCTION)

In Saccharomyces cerevisiae, Rad2p and the core TFIIH-Ssl2p complex form the NEF3 subassembly of the nucleotide-excision repair protein system.

Subject	Predicate	Object	Evidence
Rad2 NEF3 subunit	contributes to	NEF3 complex	`GO:0000112` GO:0000112 defines Rad2p as a component of the NEF3 complex.
core TFIIH complex portion of NEF3 complex	contributes to	NEF3 complex	`GO:0000112` GO:0000112 defines the core TFIIH-Ssl2p complex as part of NEF3. `GO:0000440` GO:0000440 is the core TFIIH complex portion of the NEF3 complex.
NEF3 complex	supports	nucleotide-excision repair	`GO:0000112` GO:0000112 defines NEF3 as one of several complexes involved in nucleotide-excision repair. `GO:0006289` GO:0006289 is the process term for nucleotide-excision repair. `DOI:10.1016/s0027-5107(00)00037-3` Prakash and Prakash 2000 described NEF3 as an in-vivo multiprotein subassembly of the yeast NER proteins.

Discussions

CURATION_TODO (OPEN): Resolve an exact source-neutral family grounding for the Rad2 constituent of the NEF3 complex. GO:0000112 defines budding-yeast Rad2p as the protein constituent paired with the core TFIIH-Ssl2p subcomplex in NEF3. An exact cross-eukaryotic InterPro, Pfam, NCBIfam or UniProt family boundary for this NER factor class needs narrower curation before this component label can be grounded.

Evidence

GO:0000112 Gene Ontology cellular-component term for the NEF3 complex and its exact NEF3 synonym.
GO:0000440 Gene Ontology cellular-component term for the core TFIIH complex portion that is part of the NEF3 complex.
GO:0000109 Gene Ontology cellular-component parent term for nucleotide-excision repair complexes.
GO:0006289 Gene Ontology biological-process term for nucleotide-excision repair.
PMID:10915862 PubMed identifier for Prakash and Prakash 2000, the Mutation Research yeast nucleotide-excision repair review cited by GO:0000112.
DOI:10.1016/s0027-5107(00)00037-3 DOI for Prakash and Prakash 2000, 'Nucleotide excision repair in yeast', verified through NCBI E-utilities.

Curation history

2026-09-26T02:36:48Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T02:36:48Z codex · EXPANDED_RECORD — Added NEF3 exact synonymy, nucleotide-excision repair complex parentage, core TFIIH has-part, Rad2 and core-TFIIH constituent rows, budding-yeast scope, a nucleotide-excision-repair function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a Rad2 subunit-grounding TODO.
2026-09-26T02:40:29Z codex · FIX_GO_LABEL — Canonicalized the GO:0000440 component and graph node labels to the issuing GO term label.

Source: data/structures/other/nucleotide_excision_repair_factor_3_complex.yaml